FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788049
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT82960.46396938786353165No Hit
GTCCATATGTATTTGGCTAAATTGGACAGTGTTTTCCCAAAAGCAAATGT42640.23847221189128484No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA29310.16392168223577766No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA25450.14233390695668854No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA24460.13679714593951284No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG21700.12136132734617451No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA20970.11727866518199445No Hit
GTGTAACTGATTCCCAAAAACGAAGAGAAATTCTTTCAAGGAGGCCTTCC20160.11274858798612342No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT19720.1102878053118231No Hit
GTATATTGCCATTACCCAGGGAGGAGCAATACAACTGGCTAACAATGGTA19620.1097285365222094No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT19450.10877777957986609No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG18960.10603736251075893No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA18060.10100394340423556No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)