FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411836
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA30260.2143308429590972No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA29260.20724786731603387No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT23800.16857482030490795No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG21800.1544088690187812No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC19480.13797636552687423No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT18950.13422238843605064No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG18090.12813102938301615No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA18000.12749356157514047No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA17930.12699775328012602No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG17630.12487286058720701No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG17580.12451871180505383No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC17480.1238104142407475No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT16510.11693992786697605No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA16490.11679826835411479No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC16390.11608997078980846No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC15620.11063607954464967No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT15150.10730708099240989No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA14630.10362393365801693No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)