FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615357
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATAGTTGGTTTTGGGGGGAAATTTGCAACCTCTTTATCATACCAG47240.2924430946224271No Hit
CATCATATCTGAGAAGCCATCTCTTTTGAAAGTTTCTAGTTGACATGCAG45320.2805571771441236No Hit
TCATATCTGAGAAGCCATCTCTTTTGAAAGTTTCTAGTTGACATGCAGTA42520.2632235474882642No Hit
GTATAATGAGATGGTTGATAATAGGACTTCATGACTCTATGTCTGCAAAG41740.2583948935127034No Hit
GTATTTGATTATGAAGACATTCAGATTGGATGAATTGGGAGACAGAATTT40820.25269955805434957No Hit
CACTTAGTGTTTGATGCGTTTTCATGAAGAAATGCTTCTCAAATTTGTTG34570.2140084204296635No Hit
GGTATGATCATTTCAAATTACTGATATGATACAGCAAACTGTCTTTGGAT30190.18689367118228353No Hit
CTCTTTATCATACCAGAAGTCTTCTCAACTCATCCCAAGGACTTAATGGA25700.1590979579127091No Hit
GGATAAAGTGAGAAAGTGTAAATTCTTCCAACAAGAAGCAAATTTCACAG24800.1535264340947543No Hit
GATATTACCTACCGAATTTCTAAGTAGATAACCCAGTAATCGTAGAGATA24520.15179307112916834No Hit
ATCTAGTATCTTGTTGTTGTTTTTTTTTCTTCTCATAAACTTGAAAATAA24040.14882159175959245No Hit
ATCAAATACTGCATGTCAACTAGAAACTTTCAAAAGAGATGGCTTCTCAG21480.13297370178852105No Hit
CTCTTGATCTTTACCTAAGATCAACTTTCCAGCTACCATTTTTCTATTGA19450.12040682028802302No Hit
CCTCCAGGGTCTGGGTGTCCAGCAGCAAGCCACACCAGGGGAACAGGCAG19420.12022110282742453No Hit
AACCTGAACCTACCTATTTTTTTTAAGTGCTTCAAAGAAATGTCTCCCTT19330.11966395044562905No Hit
ATATGATACAGCAAACTGTCTTTGGATCCAGCCAGACCTAGGTTCAAATA19110.1183020224012401No Hit
TCTTGATACAGAAACCTGAACCTACCTATTTTTTTTAAGTGCTTCAAAGA19070.1180543991204421No Hit
TAATAGGACTTCATGACTCTATGTCTGCAAAGAACATGCACCATGAATAC19010.11768296419924512No Hit
GTACTAGACATATTTTGCAGATAAGGAAACTGGCTGAAATATCAAGTAGC18520.11464957900946973No Hit
TCATAACGCAAACCTAATCAAATGGAAATTCTGTCTCCCAATTCATCCAA18480.11440195572867175No Hit
TTCCTGGGTCGTACCTCCAGTGTCAGGGGATCCCCCAGGGCTCCGTCCTG18200.1126685927630858No Hit
GGGTAAGGGTGTGAGGCCTGGGCTCTGGGCCAATTCTATCACCACTTAGT17750.1098828308541084No Hit
CTTAAATGCTTCTCCTAAAAAAGACAGAAAAAAGTTTCAAAAGATTGGCT17580.10883043191071695No Hit
ATATTATGTTAATTGCTCAGAAATTCAGCAAGATGGTGTTTGGGACCCGT17140.10610657582193904No Hit
GATAAATACATATTGATATGGAAAGTCTCAAAACATGATCAAATACCCTG16980.10511608269874709No Hit
TACCAGAAGTCTTCTCAACTCATCCCAAGGACTTAATGGACTTTTTCCTA16980.10511608269874709No Hit
GTCTTAAATGCTTCTCCTAAAAAAGACAGAAAAAAGTTTCAAAAGATTGG16880.10449702449675212No Hit
AAAGCAGGGTGCGTGGCATGGAGGCTCTTTAGGCAACAGCTTTGACTCTT16770.10381606047455763No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)