FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S8_R2_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S8_R2_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714717
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG36720.21414612440420194No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA29280.17075704037459244No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA26500.15454445252481896No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG21930.12789282429695395No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA20180.11768705856418289No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA18800.10963908330062627No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG18730.10923085267131544No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC18530.10806447944471305No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT18500.10788952346072267No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT17890.10433208511958533No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC17730.1033989865383034No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)