FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S7_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S7_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGG63.7267080745341614No Hit
CAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGGGCAGCT53.1055900621118013No Hit
GTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAAC42.484472049689441No Hit
CAATTACACAAGGGGCCGACTCACTGTGACCCTGACCAGGGAAGATGGAG31.8633540372670807No Hit
GGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATT31.8633540372670807No Hit
ACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACA31.8633540372670807No Hit
GTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGT31.8633540372670807No Hit
GAAAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTC21.2422360248447204No Hit
GTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGGGC21.2422360248447204No Hit
GAGTAGCACGGGGCCCGAGGAATCCCGTGTGAATCTGCCAGGACCACCTG21.2422360248447204No Hit
CCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTT21.2422360248447204No Hit
CGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGTATGCGCTTCTTC21.2422360248447204No Hit
GTTGAAGGGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGGTTTGATAC21.2422360248447204No Hit
GCCCACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGAC21.2422360248447204No Hit
GTACTGATTCACTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGT21.2422360248447204No Hit
AGCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAA21.2422360248447204No Hit
CCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCTGACCAGGGA21.2422360248447204No Hit
GTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTA21.2422360248447204No Hit
GGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCC21.2422360248447204No Hit
GGGTCACAGTGAGTCGGCCCCTTGTGTAATTGCTGTCTCTTATACACATC10.6211180124223602No Hit
GTGATACCCGGCAGGGGTTGCATGTGCGGTGTTGTGGGCCCACTCTGGGC10.6211180124223602No Hit
GTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGGGCAGCTCC10.6211180124223602No Hit
GTAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCT10.6211180124223602No Hit
GCAGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAG10.6211180124223602No Hit
TAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCATCTA10.6211180124223602No Hit
GCGGAATCACCCATTTGAAAGAGAAACCGTTATGGCAGCATTCAGCGGGA10.6211180124223602No Hit
GTCCCGTTTTCTTTTCAATACCAACACTACGTCCCCTTGCCCAATTAGCA10.6211180124223602No Hit
TGAAGACGCCGACAACGTCCTCGGGCGCCGCCGCCCGCAACGCCCGCGCG10.6211180124223602No Hit
GGGGCCGACTCACTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCT10.6211180124223602No Hit
ACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTGTCTCTTATACA10.6211180124223602No Hit
TGGTTGACCGTAGAGGGTGAGAGTCCCGTAGTTGTAATGCGTTCCGCCGC10.6211180124223602No Hit
GAGCTGCCCACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACT10.6211180124223602No Hit
GTAGTAATCATTGTTACTTCTTGCCCTTCACATCCAATTAGATAGCAACT10.6211180124223602No Hit
AAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTCTGTCAC10.6211180124223602No Hit
GCAAAAGCAGGCTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCC10.6211180124223602No Hit
GAATTAGAAGGAACAGATTTTTATCGTAATGTTCTAATGAAGATTGATTA10.6211180124223602No Hit
GGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGGTTTGATACCCAACCC10.6211180124223602No Hit
ATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAAT10.6211180124223602No Hit
GGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCC10.6211180124223602No Hit
GTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGCTGTCTCTTATACA10.6211180124223602No Hit
GGGACGTGCTGTTCGACGTCGATTGGCAGGGCGCGCGGGCGTTGCGGGCG10.6211180124223602No Hit
CCCCTAATTGCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCAC10.6211180124223602No Hit
GTGTAATTGTTGAAGGGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGG10.6211180124223602No Hit
TTTAACGGTCGGAGCCACGCAGTGAATGTACTGGAATGTCGTCAAGTACA10.6211180124223602No Hit
AGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTA10.6211180124223602No Hit
AGGAAGCTCTCGTCCAAAGCCAGTCTAGTTTCTTTGATGCCGAAAATGTG10.6211180124223602No Hit
CTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCTGACCAGGGAAG10.6211180124223602No Hit
CGGTACTGATTCACTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTG10.6211180124223602No Hit
CCTTGAGTTGCAGGTTGTCGCCGCCGAGGTTCTTCAGCAGGTTGTCCCAG10.6211180124223602No Hit
CTGGAACGGGGAATATTACAGCGAGGAGGCACTTCCACAACTACCATGTC10.6211180124223602No Hit
ATACCCGGCAGGGGTTGCATGTGCGGTGTTGTGGGCCCACTCTGGGCGTG10.6211180124223602No Hit
AAGCAGGCTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCG10.6211180124223602No Hit
CTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACCTGTCTCTTATA10.6211180124223602No Hit
GGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCTTT10.6211180124223602No Hit
GCATAAACACTGCATTCTGCCAGTGGACAGTACTGATAAAATTACCCTTT10.6211180124223602No Hit
TTAGATGACGTACTTGAAAATATGCTCCCATTACTGATGCCTATGTGAGT10.6211180124223602No Hit
GGTTGGAGATGTTGAAGGAGTTCTTTGGGAGCCCCCGGTCTGACAGCCTG10.6211180124223602No Hit
CTACGACGTTGCCCCGGGCAAGACCGCAGTTTTGCAGGCCGGTTGGATTG10.6211180124223602No Hit
ACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGGAGATTAGTGGGCAGC10.6211180124223602No Hit
GTAGAAGAGCGTCCACCCAAGCCTGCCATCCACCCATGCTGAGCTATGCT10.6211180124223602No Hit
CTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAATTACACAAGG10.6211180124223602No Hit
AGCGAAAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGA10.6211180124223602No Hit
AAATCCAAGTGTGATAAGTATAAGATTAGGTTTGTTTTGATTTATCTGTT10.6211180124223602No Hit
GGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAACCTGTCTC10.6211180124223602No Hit
ATGATAAGCTGCTTGTAGTACTCGGCAGCACCTAGGATCTCCAGTACTTA10.6211180124223602No Hit
GCAGAGAGCCCGAGGGGGGGCTTGATCCCATGACCCTGAGATCATCACTA10.6211180124223602No Hit
ATCAAACCCACTACAGCGCCAGGAGCTGCCCACTAATCTCCCTTCAACAA10.6211180124223602No Hit
CTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCCTG10.6211180124223602No Hit
TTACACAAGGGGCCGACTCACTGTGACCCTGACCAGGGAAGATGGAGATG10.6211180124223602No Hit
GTGTAATTGTTGAAGGGAGATTAGTGGGCAGCTCCTGGCGCCTGTCTCTT10.6211180124223602No Hit
ATTCTAAAGATGACAAGGTGAATCTTATTCACATTGCGGTTTGCAGATTG10.6211180124223602No Hit
TTGTTGAAGGGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGGTTTGAT10.6211180124223602No Hit
AAGCAGGGGTCAAGTGAAGAAGCGCATACGGTGGCTGTCTCTTATACACA10.6211180124223602No Hit
GCTCCTGGCGCTGTAGTGGGTTTGATACCCAACCTGTCTCTTATACACAT10.6211180124223602No Hit
GAAAGCAGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTC10.6211180124223602No Hit
TGGTTCTTGATGTGGCAGTAGAAGCAGGGGAGCGCGATGATCTTGTTACC10.6211180124223602No Hit
ATTGCGGAGTTTGCTCAGGCGGTCAAACCCGAAATTCGGGAGATGCCAGA10.6211180124223602No Hit
GACCAGGAGTGGAGGAAATACCAATCAACAAAAGGCATCCGCAGGCCAGA10.6211180124223602No Hit
CACTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCCC10.6211180124223602No Hit
CTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGCTGTCTC10.6211180124223602No Hit
TCTGACTGCCAAGGCATCCACCGTATGCGCTTCTTCACCTGTCTCTTATA10.6211180124223602No Hit
GAGCGAAAGCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTG10.6211180124223602No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA10.6211180124223602No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA10.6211180124223602No Hit
GAAAAAGTAGACGTAATGCTGCTATGACATGACATATTCCTCCAGAAACG10.6211180124223602No Hit
GCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAA10.6211180124223602No Hit
TCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGTGTAATTGTTGAAGGG10.6211180124223602No Hit
GGGGAGCCGGGCTCCTCTATGAACTGCGAGGGTAAAAGTTCTCGACTAGA10.6211180124223602No Hit
ACCGTAGAGGGTGAGAGTCCCGTAGTTGTAATGCGTTCCGCCCCTGTTGA10.6211180124223602No Hit
AAACCGTATGTGTGTGATACCCGGCAGGGGTTGCGTGTGCGGTGTTGTGG10.6211180124223602No Hit
GTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTTGT10.6211180124223602No Hit
GGCCCAGGCCAGCCGGTAGGGGCCCGACTCCTGTCGACTCCTGAGCTTGA10.6211180124223602No Hit
CAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGCTGTCTCTTAT10.6211180124223602No Hit
ACCGTAGAGGGTGAGAGTCCCGTAGTTGTAATGCGTTCCGCCGCTGTTGA10.6211180124223602No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT10.6211180124223602No Hit
GACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCACCGAAAATC10.6211180124223602No Hit
GACTATGACGGTGTGAGACCAAGAGCAGGGCGACATTAATCCAATAACTC10.6211180124223602No Hit
CTACCACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCGTATGCGC10.6211180124223602No Hit
GGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCC10.6211180124223602No Hit
CAAGGGGCCGACTCACTGTGACCCTGACCAGGGACTGTCTCTTATACACA10.6211180124223602No Hit
GGCACATGGCGCGCAGCATCGACGCTGAGGTGATCCTGGTGTCCGCACCG10.6211180124223602No Hit
GTATATTTTCATTTACATCTCTAGAAGAAGTAAAAAAGAAGAAGAGAGAA10.6211180124223602No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA10.6211180124223602No Hit
GTGAAGAAGCGCATACGGTGGATGCCTTGGCAGACAGAGGCGATGAAAGA10.6211180124223602No Hit
ACTCACTGTGACCCTGACCAGGGAAGATGGAGATGACACTCCTGCTTCTG10.6211180124223602No Hit
GGAGATTAGTGGGCAGCTCCTGGCGCTGTAGTGGGTCTGTCTCTTATACA10.6211180124223602No Hit
GAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGTGAGTCGGCCCCTT10.6211180124223602No Hit
AATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAA10.6211180124223602No Hit
GAGTAGTATCCATCATCTCAGTGAATTCTTGCTGAATGAATGAGGGATTG10.6211180124223602No Hit
CACCGGGAGTCCTCAAGCTGGAGTTTACATACGTAATTCTAATAGAAGAT10.6211180124223602No Hit
GAATCAACCCGAGTACAGGGGAATGGAATGGAATGGAATGCAATGGAATG10.6211180124223602No Hit
CTAATCTCCCTTCAACAATTACACAAGGGGCCGACTCACTGTGACCTGTC10.6211180124223602No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCTTCT10.6211180124223602No Hit
CAATTATGATCCCCATAATATGTATAAGGAATAAACGATACTATATTACT10.6211180124223602No Hit
CTCAACAGCGGCGGAACGCATTACAACTACGGGACTCTCACCCTCTACGG10.6211180124223602No Hit
AAGCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACTA10.6211180124223602No Hit
GCAGGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGCTGTCTCTTA10.6211180124223602No Hit
AAAGCAGGGGTTGGGTATCAAACCCACTACAGCGCCAGGAGCTGCCCACT10.6211180124223602No Hit
CACTATGGAAAGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATC10.6211180124223602No Hit
AATCTGTGTAAAATTACACACACTCGATCCCAGATTCCATTCCATTCAAG10.6211180124223602No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACCTGTCTCTTATACACA10.6211180124223602No Hit
ATGGAATAGACGGGATATTCCTCCAGCCTGTGGCAAGGATAAATTTTGTG10.6211180124223602No Hit
GTAGCACGGGGCCCGAGGAATCCCGTGTGAATCTGCCAGGACCACCTGGT10.6211180124223602No Hit
CTACCTGTTGACCGATAGTGAATCAGTACCGTGAGGGAATGGTGAAAAGT10.6211180124223602No Hit
GGATTTATGTGCAACTGATCCCCTCAGAGTGAGTGCTGACCGTGCCAGGA10.6211180124223602No Hit
GCGAAAGCAGGGGTTGGGTATCAAACCCACTACCTGTCTCTTATACACAT10.6211180124223602No Hit
CTCGGGCCCCGTGCTACTCGGGCAGCCACTCAACAGCGGCGGAACGCATT10.6211180124223602No Hit
GGAGTGTCATCTCCATCTTCCCTGGTCAGGGTCACAGCTGTCTCTTATAC10.6211180124223602No Hit
GAATAGTCTAGAGCAGAGAACAGGATTGGGTATTCTAAATCCTTTTATCT10.6211180124223602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)