FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S7_R2_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S7_R2_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTAGATGATGAATGAGATGATGATGAATTAGATGATGATGATGATGA33.7037037037037033No Hit
GTTCCGGGGACTCCCTGGACGTGAAATTCATCGTCACCGACTTCAACAAA22.4691358024691357No Hit
CTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTCTTTTCAT11.2345679012345678No Hit
GCCTCAGTCTTGGCTCCTCATAAAGTTGTGTGGTTGGGGGCTGTCTCTTA11.2345679012345678No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAGTGGCGAGAGC11.2345679012345678No Hit
AGATGATGATGAATTAGATGATGATGATGATGATGACGAAACGCTTGGAC11.2345679012345678No Hit
GACCAATGTCAAGAGACTTCTTCCCTGTTTCCTTCTAGGCATTTTGTCAT11.2345679012345678No Hit
AACTGAGCCTGGTTTCTCCCAGCAACCGGTCATACACACCAAAGATGGCC11.2345679012345678No Hit
ATACAAAGAACAGATAGGAAAAAAGCTTAAAAAAAAAATTAAATTTAACT11.2345679012345678No Hit
GGCTATTTTCATCTGAATGGGATAGGGGTGTTAGCCCTGTCTGGGAAGGC11.2345679012345678No Hit
AAACAGAACGGTATAAAGTCAATGAATTTAACCAAGTGACGGGTTACGAA11.2345679012345678No Hit
ACGAATACCTATTTGAGCCCCTGCTATTAATTCATTTGGAAATAACACAC11.2345679012345678No Hit
GTTGCACCACCAGCGCCTGCCACGGAAAAGGTCGCGAGCAAGAAGTTCGA11.2345679012345678No Hit
GAAGAAGCTAGAAAAGTAAGGTAGATGATTAGTAGCTATCATTGATAATT11.2345679012345678No Hit
ATGATGATGATGATGACGAAACGCTTGGACTAGTAGGAGAGAGTGGAAGA11.2345679012345678No Hit
CCCTTCGGTGTTTGACAAGTTGTATTGCAATAGTGGACTGGTGTATCTGA11.2345679012345678No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG11.2345679012345678No Hit
AAATTAATCTGAAATAGTTAAGGACTTAACCATACGACCCAAGATGACAA11.2345679012345678No Hit
ATCTCGGTGGCATCCTGCTGCTGCTGATCGTGTTCGTGGGCTTCGTCTCG11.2345679012345678No Hit
ATACATAACCAGGTGGTGTTTATCTCCAACCTTTTTTTAATGCGCCAGGA11.2345679012345678No Hit
AGCTCATCCTTCTCCCTTTCTTCAGCATTGCTGGTGAAGGTGGTAATGGG11.2345679012345678No Hit
GGCACATAACTAAATACTTAATTTCTGTCTGATTTTTTTTTTTGCAATCT11.2345679012345678No Hit
GAATTGGCCTGTTCATCGCTTTTGTGGGATTTGTGGATTCTGGCGTGATT11.2345679012345678No Hit
TTATTTCTCTGTTGAGCTTCTTGTATAGTTTAACTGCTATATCCATGTTG11.2345679012345678No Hit
CGCAAGGAGGGCCTAGTATCTTTTGCCTAGGCATGAGCATGAACCAGTCT11.2345679012345678No Hit
ACTTAACAGCCAGGAGCAAGTCCAATTAATTATTTCAGCAAAAATTTAGA11.2345679012345678No Hit
TCCTTAAGAGAAGTTATGTCATTTATGATAGTCTAAACAATAAGCTTAAG11.2345679012345678No Hit
GAGCAAAAGCAGGGAGAGTGGGAGAGGGAGAAGCAGGCTCCCGCTGAGCA11.2345679012345678No Hit
GTATTACTACTGAAAATGAAAAGAATTTGAAACAAAGTGATAAATAGCTG11.2345679012345678No Hit
ATGAAGACCTCTCTATTATTCGGTGTGCCTGGCGCGGTGATTTTCTTTAT11.2345679012345678No Hit
GTTAGTGAAGGTATCCCATTCTCATAACAGTTTCTCCAAGCAACACTCAG11.2345679012345678No Hit
CACGAGGACGGTCCGGGCGGCATCTCCATGCCACCCATCAACAATGGCGC11.2345679012345678No Hit
AATCATAACTGATCTTTGCAAAAACTCACTTAGCTAGCATCTCATGTTAG11.2345679012345678No Hit
ATACAGAATTTAAATAAAAAGGTTGAAGATGGTTTCCTGGACAATTGGAC11.2345679012345678No Hit
TTTCCAACCTGAGCGATCGTCTGATCCTCACCCTGACTTCTGATGCACGG11.2345679012345678No Hit
CTCCGGGCTGAGTAAGGAGCCTCAAATGCAGCTCAATCACTAGCCTCCGG11.2345679012345678No Hit
GAAAGAGGAATCATGCGAGACAATTAGAATGACACTACAATCTGTCCCTA11.2345679012345678No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGATCT11.2345679012345678No Hit
CGCATATAGACTGTTGAATACAGATTTTGCAAGTAAGGTACTGCACACTT11.2345679012345678No Hit
GAAGAGAAGGTAATGGTGAAATTTCTCCAACTAATGCTCCCTCCTCAGTG11.2345679012345678No Hit
TAGCAGGATTGGGCATTACAACCTTAATGCTGCAACCTATCAGAGACCAA11.2345679012345678No Hit
AGGTACGGATATAGTGTATCTTGACTGAAGGGATGAACTCTGTCCACTGC11.2345679012345678No Hit
ATTACAAAATGTGAAATAGCAGCAAGTGATGAATTCTCTCCCAAGACACC11.2345679012345678No Hit
TATCATCACCAGCCAGATTGAACACAAGGCCACGTTGGATACCGCCCGCC11.2345679012345678No Hit
CCAAAAGTGGTCTAATAATCTCAATTAAATACTCTTACCGTAAATAGAAA11.2345679012345678No Hit
GTCTCTAATAGGTTAAATAGTACACTGCAGTTCGCTTTCAGTACGATGTT11.2345679012345678No Hit
GTATCGAAGTGAACCCTCCTAGGAGTCTTCACACTCCCCTGCGGAAACAG11.2345679012345678No Hit
GTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTC11.2345679012345678No Hit
GTTAATTAAAATACAAAAATGAAAATAAAAAGAAAATTAGATCCTACAGC11.2345679012345678No Hit
GCAATGGACCAGTTTCACACTCTGAGTCCATTCTCGGAACCCACACTGAC11.2345679012345678No Hit
GTGCAAGTTCAGGGGTGAATTCAGGGCTTGAATTTTCGAATTTGGAAGTA11.2345679012345678No Hit
GTTTATAGCACCCTTGGGTGTTTGACAAGTTCTATTGCAATCGTGGACTG11.2345679012345678No Hit
CCAGACATATTACGAACACATACATGCAGTTTTGTTACTCATCGTAGGAA11.2345679012345678No Hit
CCAATATCCTTGGAATTATTTTATAGCTGGCTTCTTACCATTACATATAA11.2345679012345678No Hit
GGGTTCATGACCTGTCCGTACCGCGGCCTACAATCACTGACCCAGCCACA11.2345679012345678No Hit
AGAATGATCCAGGAAGGGGAGACACAGATTTGCACCTCATCCCCCGTAGC11.2345679012345678No Hit
GGTTTGAGTACGCGCCCTGAGCAACCGGTGGCGGTGGCGGGCGTGCAGAC11.2345679012345678No Hit
ATCTCAAACAAATGAAACATTGAGAGACCACAATCAACAAATTATTTAAA11.2345679012345678No Hit
ACATTACTCATGTCAAAGCAAGGCACGATCGGGTTCGTTGCCTTTTCGTC11.2345679012345678No Hit
CCAGCGTGTGCCGGGATGGTCCCGAAGGAGTCATGAATCAGGGCAAAGTA11.2345679012345678No Hit
GTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG11.2345679012345678No Hit
GCTAGTCACTAAGCAGCAGGGTGAAGACAATCCCCAGTAAGATCTCTTCA11.2345679012345678No Hit
ATACAGCGCCGCCGCTTCGCGGGGCCGCTGTTGTTCCAGTGCCTCTGTCT11.2345679012345678No Hit
GAACCCACCAAAACTGAAAGATGAGCTAATCCTCAAAACTATTGCTGCCT11.2345679012345678No Hit
CCAAGGAGAGAGCCCGATGTTGGTCTCGATACACGGGCCTGGGGACCTTG11.2345679012345678No Hit
GCGCTGACCCACCATCGGGCACTATTGTCATCCTCATTACTTGTTCCTCG11.2345679012345678No Hit
GGGTGGGGCGTCTGCTGGTTGGGTGGGAGAAGAGAACCGGCTGTTGGCAG11.2345679012345678No Hit
ACACTGAACCTTGGACAACATGGCAACCACAAGGTTTCTTGTCGTGGATA11.2345679012345678No Hit
TACTAGGACAGCATTTATTTTGCTACCAGTACAAGTTCTGAATAGTGCTG11.2345679012345678No Hit
GTACTCTCACCGGTTAATAAGTACTCTTTGCCATTTAAGGACCTATGAAT11.2345679012345678No Hit
TTAAAATACTATCCAAAGATGATCAGCTTTATAACTGTGCATGTGGTCGA11.2345679012345678No Hit
CTGGTGGACCTAATCCCGCTCTTTCTGCATGACAAATGGAGTCAGTTGCT11.2345679012345678No Hit
CAATAAAACACAAGTTATAATATTTAACATAGCTATAAAACTCAAAACAT11.2345679012345678No Hit
CTCATCTAAAACTTCAAGCAGTAGTAGTAATCAAAGCAAAAAAATTAATA11.2345679012345678No Hit
GCCCAGCAGCAGCGGGAAACCTTGCTGCGCTTTCTGTCCCACGACCTGCG11.2345679012345678No Hit
GGGTTGATAATCACTCAATGAGTGTCTACGATGCCATGGCGTCTCAAGGC11.2345679012345678No Hit
GCTTGATCGCCTCCAGGTTGTTGATCACCACCTTGACCGCATCGCTCCCC11.2345679012345678No Hit
AAGTAGAACTAAGATCATAAAAATTAAGAAAACCAAAAAAAAATTACAGT11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)