FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S7_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S7_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484312
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG36530.24610728741666174No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC32860.22138202749826183No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA32650.21996723060919804No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA30830.2077056575706455No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28300.19066072362144887No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT28210.1900543820975644No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT27420.1847320509434674No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG27220.18338462533483527No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA26020.17530007168304237No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA25340.1707188246136931No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT23880.1608826176706784No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC23190.1562339993208975No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT22190.14949687127773675No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT21040.14174917402810192No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20850.14046911969990136No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20030.13494467470450958No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA19580.13191296708508723No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA18960.12773594769832758No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18590.1252432103223581No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG17890.1205272206921456No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC17660.11897768124221862No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT17180.11574385978150148No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG17040.11480066185545895No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT16600.11183632551646824No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT16570.11163421167517341No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG16150.1088046178970459No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC15760.10617713796021322No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT15640.10536868259503393No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT15430.10395388570597017No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC15390.10368440058424375No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)