FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S7_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S7_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296189
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGACAGAGTCTGGCACCCAGAGACCCCTCTCCGTGTGGGGTATCTC5930.20021000104662903No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3920.13234792649288124No Hit
CTGTCTACACAGAGCGGAGCCTAAGCAAAGCTCTGAAATCTCTGCAAACA3760.1269459703094983No Hit
GTGTAGGCCAGGGCGTTGGTTAACACTGGACAGGAGTCCCAAATGCCAAA3700.12492023674072973No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG3340.1127658353281182No Hit
GTGCTAGAGAGAGCACAAAGACTGCAGCATTTGCAGCTGCTGAGTGAAAT3250.10972723497496531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)