FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256
Sequences flagged as poor quality0
Sequence length151
%GC66

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTC1028.121019108280255No Hit
ATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGAT584.617834394904459No Hit
GGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCA524.140127388535031No Hit
AGCGAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCC372.945859872611465No Hit
GAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCC292.3089171974522293No Hit
GCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGA262.0700636942675157No Hit
AGCAAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCC251.9904458598726114No Hit
CCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAG251.9904458598726114No Hit
GTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCA241.910828025477707No Hit
AAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCA231.8312101910828025No Hit
AGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGC201.5923566878980893No Hit
GGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCG181.4331210191082804No Hit
GCAAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCG181.4331210191082804No Hit
AAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTAT181.4331210191082804No Hit
ATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCT171.3535031847133758No Hit
GCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAG161.2738853503184715No Hit
GAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATT151.194267515923567No Hit
CATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCC141.1146496815286624No Hit
GGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGA131.0350318471337578No Hit
ATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTG131.0350318471337578No Hit
AGCGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGG110.8757961783439491No Hit
GGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCAC110.8757961783439491No Hit
AAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCG100.7961783439490446No Hit
CAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGG100.7961783439490446No Hit
GAGCGAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGC100.7961783439490446No Hit
CAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCG100.7961783439490446No Hit
GCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCC90.7165605095541402No Hit
GCGAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCG90.7165605095541402No Hit
GATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGAC80.6369426751592357No Hit
ATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCT80.6369426751592357No Hit
GCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGA80.6369426751592357No Hit
GCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGT80.6369426751592357No Hit
GCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTC80.6369426751592357No Hit
GAGCAAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGC80.6369426751592357No Hit
GTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGC80.6369426751592357No Hit
GATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCC80.6369426751592357No Hit
GATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTC70.5573248407643312No Hit
AAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAG70.5573248407643312No Hit
GTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCG70.5573248407643312No Hit
CGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGC70.5573248407643312No Hit
CTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGA70.5573248407643312No Hit
GGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGG60.47770700636942676No Hit
GGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCG60.47770700636942676No Hit
GCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCG60.47770700636942676No Hit
AGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATC60.47770700636942676No Hit
GGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTG50.3980891719745223No Hit
GCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAG50.3980891719745223No Hit
AGCAAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGG50.3980891719745223No Hit
TCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGA50.3980891719745223No Hit
TGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAG50.3980891719745223No Hit
GGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCG50.3980891719745223No Hit
GAGCAAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCG50.3980891719745223No Hit
GGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATG50.3980891719745223No Hit
GAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGC50.3980891719745223No Hit
CCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCG50.3980891719745223No Hit
GAGCGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCG50.3980891719745223No Hit
CGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACC50.3980891719745223No Hit
GAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGG50.3980891719745223No Hit
ATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGA40.3184713375796179No Hit
GGTCAGGGAAAAGGACTTTGACATTGCCAACTTCCTGAACGTACTCGAAC40.3184713375796179No Hit
CTCCAGTCCCAGCGTTTGCCAGATTTTCGGTGCACGTTTTACGTGACCGG40.3184713375796179No Hit
GCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGC40.3184713375796179No Hit
GGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGT40.3184713375796179No Hit
GCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGG40.3184713375796179No Hit
GCGAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGA40.3184713375796179No Hit
AAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGC30.23885350318471338No Hit
CTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGG30.23885350318471338No Hit
TGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATC30.23885350318471338No Hit
GGTCTAACTGCTTGTTGGAGTTCAGCAGGTAATCATATTCGCCACCCTTG30.23885350318471338No Hit
GCAAAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGA30.23885350318471338No Hit
TTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGC30.23885350318471338No Hit
GTATATGGGCGTTGGCGGGACTTACGATGTTTTCACCGGTCACGTAAAAC30.23885350318471338No Hit
GTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCGCGGCCTGATGCAATC30.23885350318471338No Hit
CGGTAGAGCCACTCCAGTCCCAGCGTTTGCCAGATTTTCGGTGCACGTTT30.23885350318471338No Hit
AGCGAAAGCAGGTCTAACTGCTTGTTGGAGTTCAGCAGGTAATCATATTC30.23885350318471338No Hit
CGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACT30.23885350318471338No Hit
CGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCGCGGCCTGA30.23885350318471338No Hit
TGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTC30.23885350318471338No Hit
CGTTTTACGTGACCGGTGAAAACATCGTAAGTCCCGCCAACGCCCATATA20.15923566878980894No Hit
AGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTT20.15923566878980894No Hit
GCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCTGTCTCTTATAC20.15923566878980894No Hit
GAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGCGGCCTCAGTC20.15923566878980894No Hit
GACTGAGGCCGCGGATAATCCGCGGCCTGATGCAATCCGGCCCCATCCTC20.15923566878980894No Hit
AGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCG20.15923566878980894No Hit
GGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGG20.15923566878980894No Hit
AAGCAGGAGATCTTCATGCGCGACTGCCGTCTGGTGCATCCGGATGCGCT20.15923566878980894No Hit
TCTCTGAGCTGGGCATGAGGGACACACAGATGAATGCAGCACGGTAGTAG20.15923566878980894No Hit
CGTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCGCGGCCTGATGCAAT20.15923566878980894No Hit
AGGCCGCGGATAATCCGCGGCCTGATGCAATCCGGCCCCATCCTCCTGTC20.15923566878980894No Hit
CAAAAGCAGGCGATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGG20.15923566878980894No Hit
CGGATAATCCGCGGCCTGATGCAATCCGGCCCCATCCTCAACATCTCCTG20.15923566878980894No Hit
GGTAATCATATTCGCCACCCTTGTAATAGGGTTCGAGTACGTTCAGGAAG20.15923566878980894No Hit
TTACGTGACCGGTGAAAACATCGTAAGTCCCGCCAACGCCCATATACAGC20.15923566878980894No Hit
CATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGT20.15923566878980894No Hit
TCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCC20.15923566878980894No Hit
GGCCGCGGATTATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGC20.15923566878980894No Hit
AGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTATCCGC20.15923566878980894No Hit
AGCGAAAGCAGGAGATCTTCATGCGCGACTGCCGTCTGGTGCATCCGGAT20.15923566878980894No Hit
ATGAGCCATGAAGAGGCGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCA20.15923566878980894No Hit
ATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATAATCCGCGGCCTGATG20.15923566878980894No Hit
ATCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCG20.15923566878980894No Hit
AAAGCAGGAGATGTTGAGGATGGGGCCGGATTGCATCAGGCCGCGGATTA20.15923566878980894No Hit
AGCCATTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGA20.15923566878980894No Hit
TCCGCGGCCTCAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGA20.15923566878980894No Hit
GCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTCGTCGC20.15923566878980894No Hit
TTTCGATCCGCGGGTCGTCGAGGCCCTCGATGCCTGTCTCGCGGAGGTGC20.15923566878980894No Hit
GCGGAGGTGCGCGCGATCGCGTCCCGGTGGGCCGACTGAGGCCGCGGATA20.15923566878980894No Hit
GGATGAAGGCCAGCGCCTCTTCATGGCTCATCGCCCTGTCTCTTATACAC20.15923566878980894No Hit
CGCTGGCCTTCATCCGGGCCGGCAGCGGCAGCCATTTCGATCCGCGGGTC20.15923566878980894No Hit
ACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATCGAGGGCCTCG20.15923566878980894No Hit
CAGTCGGCCCACCGGGACGCGATCGCGCGCACCTCCGCGAGACAGGCATC20.15923566878980894No Hit
AGGCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCTGTCTCT20.15923566878980894No Hit
GATAATCCGCGGCCTGATGCAATCCGGCCCCATCCTCAACATCTCCTGCT20.15923566878980894No Hit
CAGGTAATCATATTCGCCACCCTTGTAATAGGGTTCGAGTACGTTCAGGA20.15923566878980894No Hit
GCCCTCGATGCCTGTCTCGCGGAGGTGCGCGCGATCGCGTCCCGGTGGGC20.15923566878980894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)