FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186515
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA41840.3526293388621299No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT38750.3265866845341188No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA35860.30222963890047744No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG31190.26287067588694624No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG27340.23042270852033056No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG25180.21221813462113837No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT22870.19274935420116895No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC21560.1817086172530478No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA21010.17707319334353128No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC20820.1754718650838801No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC20240.17058359987020813No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA19220.16198699552892293No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC19100.16097563031230114No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA17980.15153622162383115No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA15320.12911762598871485No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG14290.12043674121271118No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA14020.11816116947531215No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT13880.11698124338925339No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT13360.11259866078389233No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC13180.11108161295895963No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT12660.10669903035359857No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA12520.10551910426753981No Hit
CTATAAGGAAGAGCTATCTCTGTAATTTAAGAAGTTATTTTAAAACCAAG12520.10551910426753981No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT12510.10543482383282134No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT11920.10046227818443089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)