FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596801
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA63720.39904784628767137No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT60900.38138753670620196No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT58010.3632888506457599No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG50910.31882495063567723No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG42740.26766015301844126No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA41060.25713911752309776No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC38800.24298581977340947No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA37880.23722430033548325No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG37470.2346566666729292No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA35620.223071002585795No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG35120.21993974202170466No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA31410.1967057886361544No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA30530.19119477004335542No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA30450.19069376835310098No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA27330.17115470243317732No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC26370.1651426821501239No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC24300.15217926341478993No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG23360.14629249355430013No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA22490.14084410017278295No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG22250.1393410951020196No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT22040.13802596566510167No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG21640.1355209572138294No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA21460.13439370341075688No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT21420.13414320256562967No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA21290.13332907481896616No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC20340.12737967974719455No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA20240.12675342763437647No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG19900.12462417045079506No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT19520.12224441242208642No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG19400.12149290988670473No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT19350.12117978383029569No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC18770.11754752157595093No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC18450.11554351481493311No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT18040.11297588115237904No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA17350.10865474157393437No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC16890.10577398185497129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)