FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39207
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCA16564.22373555742597No Hit
GGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAG14833.782487821052363No Hit
GTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAG11793.0071160762108806No Hit
GAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTAC9512.425587267579769No Hit
CCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCG9222.3516208840258117No Hit
GACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTT8932.2776545004718542No Hit
TCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGA7231.844058458948657No Hit
AGCGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTAC7111.8134516795470197No Hit
GAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGG6571.6757211722396512No Hit
GTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGC6541.668069477389242No Hit
GTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGG6371.6247098732369218No Hit
GCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGG6181.5762491391843294No Hit
AGCGAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGC5751.4665748463284618No Hit
GGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTA5621.433417501976688No Hit
AAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGA5111.3033386895197288No Hit
TGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGAC5071.2931364297191827No Hit
ACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGT4641.1834621368633151No Hit
TGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGC4231.0788889739077205No Hit
CCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCC3920.9998214604534905No Hit
GGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTG3820.9743158109521258No Hit
AAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGG3690.9411584666003521No Hit
AGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATT3650.9309562067998063No Hit
CTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGG3600.9182033820491239No Hit
GCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTT3600.9182033820491239No Hit
GTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAA3160.8059785242431198No Hit
AGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATG3100.7906751345423011No Hit
CGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAA2960.7549672252403907No Hit
GATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGG2930.7473155303899813No Hit
AGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGC2920.7447649654398449No Hit
AAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTG2840.7243604458387533No Hit
AAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGAT2840.7243604458387533No Hit
GACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAA2800.7141581860382075No Hit
GTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTG2640.6733491468360242No Hit
GGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAG2630.6707985818858877No Hit
AGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGA2580.6580457571352055No Hit
GCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACT2520.6427423674343867No Hit
CTCCTGTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCCAGAGTAGTC2470.6299895426837044No Hit
GGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAA2400.6121355880327493No Hit
GGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACG2330.5942816333817941No Hit
GCGAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCT2290.5840793735812483No Hit
AGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAG2270.5789782436809754No Hit
CTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTT2260.5764276787308389No Hit
GGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCT2190.5585737240798837No Hit
GCGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACT2180.5560231591297472No Hit
CTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCC2160.5509220292294743No Hit
GCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGA2100.5356186395286556No Hit
GAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTG2040.520315249827837No Hit
GCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTG2030.5177646848777003No Hit
GGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCA2010.5126635549774274No Hit
GTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCCAGAGTAGTCTCTTC1980.5050118601270182No Hit
TCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTCA1900.48460734052592647No Hit
CAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAG1890.48205677557579No Hit
ATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGC1870.4769556456755171No Hit
CTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGG1860.4744050807253807No Hit
GTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGC1840.4693039508251078No Hit
GAGCGAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTG1720.43869717142347026No Hit
GTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCA1690.4310454765730609No Hit
GCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCT1570.40043869717142344No Hit
CTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCC1540.3927870023210141No Hit
CAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTC1540.3927870023210141No Hit
GGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGA1520.3876858724207412No Hit
TTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGC1490.38003417757033187No Hit
CAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGT1490.38003417757033187No Hit
CAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGC1440.3672813528196496No Hit
CAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAA1440.3672813528196496No Hit
AAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGG1430.3647307878695131No Hit
ACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGC1400.35707909301910373No Hit
GCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAAT1370.34942739816869434No Hit
CCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATG1340.341775703318285No Hit
CTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCC1290.32902287856760276No Hit
GCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGA1290.32902287856760276No Hit
GTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTA1280.3264723136174663No Hit
AACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACA1240.31627005381692047No Hit
TGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGT1220.3111689239166475No Hit
ACGCAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGG1190.3035172290662382No Hit
AGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTA1180.30096666411610173No Hit
GCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAGGGGTTAACGA1170.29841609916596523No Hit
GCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCC1160.2958655342158288No Hit
GTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTA1150.29331496926569234No Hit
GTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAG1140.2907644043155559No Hit
CTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAA1100.28056214451501005No Hit
GCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCA1100.28056214451501005No Hit
CACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGAGC1090.2780115795648736No Hit
CTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGT1090.2780115795648736No Hit
CGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTCAGCCTACCCTACTGCC1090.2780115795648736No Hit
CTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCT1060.27035988471446426No Hit
GAGCGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTA1020.2601576249139185No Hit
CAGCAGGGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGA1020.2601576249139185No Hit
GCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCA1010.25760705996378197No Hit
CAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAG1000.2550564950136455No Hit
AGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAG990.2525059300635091No Hit
GCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGG970.24740480016323618No Hit
GAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCT960.24485423521309968No Hit
CTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTT960.24485423521309968No Hit
TTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTG950.24230367026296323No Hit
TCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAA940.2397531053128268No Hit
AGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACA940.2397531053128268No Hit
GCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAA900.22955084551228097No Hit
GGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGG890.22700028056214452No Hit
GTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGG890.22700028056214452No Hit
TAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGC880.22444971561200808No Hit
CGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTA870.22189915066187163No Hit
GACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGGAG830.2116968908613258No Hit
GAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCT810.2065957609610529No Hit
GAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGC810.2065957609610529No Hit
CTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTG790.20149463106077994No Hit
CATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACT780.1989440661106435No Hit
AGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCG780.1989440661106435No Hit
GGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAG780.1989440661106435No Hit
GGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGG780.1989440661106435No Hit
CTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTG770.19639350116050705No Hit
ACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAA760.1938429362103706No Hit
CAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCC720.1836406764098248No Hit
GAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCAC710.1810901114596883No Hit
ACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCT700.17853954650955187No Hit
GCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTA690.1759889815594154No Hit
CAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCAT690.1759889815594154No Hit
TAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCT690.1759889815594154No Hit
GGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAA680.17343841660927894No Hit
GTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGT660.16833728670900605No Hit
CAAGTGGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAA640.16323615680873316No Hit
GGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAG630.16068559185859668No Hit
AGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCA600.15303389700818731No Hit
CTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGTCAG600.15303389700818731No Hit
GGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTTTCCTAGCATCT590.15048333205805087No Hit
CCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTAGTCCCTGCTGCGT580.1479327671079144No Hit
GGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGAGCTCTAGC580.1479327671079144No Hit
AGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTACTA560.14283163720764147No Hit
AGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCA560.14283163720764147No Hit
AGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGT560.14283163720764147No Hit
CCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGC540.13773050730736858No Hit
GACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGA540.13773050730736858No Hit
CTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCTCCTGTAC540.13773050730736858No Hit
GAGCTCAGGAACAAAGTTAAGGCCAAGTGGCCCGGAAGCAAAGGCCCTTG520.13262937740709568No Hit
GACGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACG520.13262937740709568No Hit
GAAAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAG500.12752824750682276No Hit
GGACTAGTACAGGAGGGGTTAACGAGCATTGCTTCAAGAGGTGAGAGTTT480.12242711760654984No Hit
CGAAAGCAGGGAAGTGATCGTGACGGAAGCACGCGCAGAAGAGACTACTC480.12242711760654984No Hit
AGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTG470.1198765526564134No Hit
ACACAAGGGCCTTTGCTTCCGGGCCACTTGGCCTTAACTTTGTTCCTGAG460.11732598770627695No Hit
GCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAACCCCT460.11732598770627695No Hit
AGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGAACAAAGTTAAGGCC450.11477542275614049No Hit
CAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCT440.11222485780600404No Hit
GAGACTACTCTGGCAGTAGGGTAGGCTGACGCAGCAGGGACTAGTACAGG440.11222485780600404No Hit
GGCCCGGAAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTC440.11222485780600404No Hit
CGGAAGCACGCGCAGAAGAGACTACTCTGGCAGTAGGGTAGGCTGACGCA420.10712372790573112No Hit
CAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAGCTCAGGA420.10712372790573112No Hit
CAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAA410.10457316295559467No Hit
TGCTTCAAGAGGTGAGAGTTTTCCTAGCATCTGCAGGGACACAAGGGCCT410.10457316295559467No Hit
AAGCAGGCAAAGACCCGCGTCAGCAAGTGTCCCTCTTAGGTGTGCTAGAG400.1020225980054582No Hit
GTTTTCCTAGCATCTGCAGGGACACAAGGGCCTTTGCTTCCGGGCCACTT400.1020225980054582No Hit
CCCTGCAGATGCTAGGAAAACTCTCACCTCTTGAAGCAATGCTCGTTAAC400.1020225980054582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)