FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608472
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGATAGTATGTGTCTTTTCATTGGTACATTTAAACCATTGTTGCAAA115650.719005366583938No Hit
ATCTAATAGATAGATAACATTCTAAGAAAGGAGCAAAATTAGAATCATAT67800.4215180618624384No Hit
ATCACACACTGCGCATCGCGCATTCTATAATTTCTATTCATTGAAGGATG59210.3681133398654126No Hit
ACACATACGTATCTAACAGATAAATCTAATAGATAGATAACATTCTAAGA42980.267210122401882No Hit
GATAGTATGTGTCTTTTCATTGGTACATTTAAACCATTGTTGCAAAAAAA41960.2608687002322701No Hit
CTCTAAACCCATTCAACCATTCAGCCCTTTGCTTTCTGATAAGACTGCCA41070.25533549853525583No Hit
CATATAAAATGCTCAGCTAAAACCACAAAAGGCAAAAAGAAAATGGAAGA40260.250299663282917No Hit
ATAGATAACATTCTAAGAAAGGAGCAAAATTAGAATCATATAAAATGCTC38660.24035233438940812No Hit
GGCTAAACCTGCTCTCCTTCCAAAAATAAAAGAAATCCTCCGTAGAAACA37650.23407308302538063No Hit
GTTTAAAATATAATGGCTAAACCTGCTCTCCTTCCAAAAATAAAAGAAAT35140.21846821082368859No Hit
CTATAATTTCTATTCATTGAAGGATGAGGTCAGAATGAAGTGTTCCCGTT32050.19925743189809958No Hit
CTAATAGATAGATAACATTCTAAGAAAGGAGCAAAATTAGAATCATATAA31710.19714362450822892No Hit
CTTCTGTAGCGTGTGCACCTGCCTACCAGTTAGCTTAGTGGGCAGAAAGA31210.1940350842290074No Hit
AATATATACATATGACACATACGTATCTAACAGATAAATCTAATAGATAG31060.19310252214524093No Hit
GCTAAAACCACAAAAGGCAAAAAGAAAATGGAAGACAAAATAGGAACAAA28610.1778706747770555No Hit
ATTTAAACCATTGTTGCAAAAAAAAAATGCTTATTGATGTAATTGAACTT28520.17731113752679561No Hit
GTATGTGTCTTTTCATTGGTACATTTAAACCATTGTTGCAAAAAAAAAAT28520.17731113752679561No Hit
GTCTCAGGACCCTGGGATCATGAGCTAAGCCAGAGGCAGGTGCTTAACCA28520.17731113752679561No Hit
TCTGTAGCGTGTGCACCTGCCTACCAGTTAGCTTAGTGGGCAGAAAGAAG27970.17389174321965195No Hit
GTCTATAGTTTACTCAGTAATGCCCCCAAAGAAAGAAACTAATGTCTCTT27650.17190227744095019No Hit
ATAGTAACCAATGTGGTAGATAGAAGTTCAATTACATCAATAAGCATTTT27510.17103188616276815No Hit
AGCAGGAGGAGTGGGAGAGGGAGAAGCAGGCTTCCCACTGAGCAGGGAGC27130.16866939555055976No Hit
ACATATGACACATACGTATCTAACAGATAAATCTAATAGATAGATAACAT26960.16761249185562446No Hit
CATTCTAAGAAAGGAGCAAAATTAGAATCATATAAAATGCTCAGCTAAAA25700.1597789703519862No Hit
CTCCTATATTTATTATAGATTCGATTCTCCCTAGTTATGAAACTTAAGGT23520.1462257347345804No Hit
GTAATCACACACTGCGCATCGCGCATTCTATAATTTCTATTCATTGAAGG23070.14342804848328103No Hit
GCCAACAACTATGCCAATTAGTGTCAATTGCGATGTTCGCTTCTGTAGCG22650.14081687464873496No Hit
GATAACATTCTAAGAAAGGAGCAAAATTAGAATCATATAAAATGCTCAGC22410.1393247753147086No Hit
ATTTATTATAGATTCGATTCTCCCTAGTTATGAAACTTAAGGTTTTTAAA22360.13901392128678647No Hit
CATATGACACATACGTATCTAACAGATAAATCTAATAGATAGATAACATT22210.13808135920302No Hit
GAGTGGGAGAGGGAGAAGCAGGCTTCCCACTGAGCAGGGAGCCCAATGAG22200.13801918839743557No Hit
GTAAAAGGTTACCTGAAAAATAATCAGTAATCACACACTGCGCATCGCGC22010.1368379430913314No Hit
CCTATATTTATTATAGATTCGATTCTCCCTAGTTATGAAACTTAAGGTTT21820.13565669778522724No Hit
GTCTTAGGCCACCCCCTGGGCTGAGAGGAAGCAGGAGTGTGGACAGAAAG20710.12875573836535545No Hit
GTATCTAACAGATAAATCTAATAGATAGATAACATTCTAAGAAAGGAGCA20610.12813403030951115No Hit
CTACAGAAGCGAACATCGCAATTGACACTAATTGGCATAGTTGTTGGCAG20030.12452812358561417No Hit
CCCTAGTTATGAAACTTAAGGTTTTTAAAAAAGTGTTACTGTTACAGTTT19510.1212952416952238No Hit
GATAAATCTAATAGATAGATAACATTCTAAGAAAGGAGCAAAATTAGAAT19420.12073570444496391No Hit
GGTTACTATTTTCTATTTCTTGCTATTGTTCTTTGTTCCTATTTTGTCTT18960.11787584738808012No Hit
GTTCTAGAGGCTGAAAGTCCAAGCGAGCTCAAGGTGCTGGCAGGGCTGGT18610.11569986919262505No Hit
CATCTATGGTGACTGTAGGGTCTTGTTACCATAGTCTGCTTTTATGGATT18520.11514033194236518No Hit
AAATTAGAATCATATAAAATGCTCAGCTAAAACCACAAAAGGCAAAAAGA18470.11482947791444302No Hit
CATTTAAACCATTGTTGCAAAAAAAAAATGCTTATTGATGTAATTGAACT18400.114394282275352No Hit
AATTAGAATCATATAAAATGCTCAGCTAAAACCACAAAAGGCAAAAAGAA17930.11147225441288378No Hit
GGCTTCCCACTGAGCAGGGAGCCCAATGAGGGACTCAGTCTCAGGACCCT17550.10910976380067543No Hit
CTATCAGAATGGTCAATAAAAAAGATTAAAAAAGGGGGTGCCTGGGTGGT17390.10811503091132453No Hit
CCCAAGAAGAGTATCCTAGGCTTTACTTACTAGATTCCAATAGCACCTCA17290.10749332285548024No Hit
ATAGTAAGTTAGCTCAGAATGCCCTAACAAAGTACCGGGACTGGCTGGCT17090.10624990674379163No Hit
GAGCAGGGAGCCCAATGAGGGACTCAGTCTCAGGACCCTGGGATCATGAG16830.10463346579859642No Hit
GAACAATAGCAAGAAATAGAAAATAGTAACCAATGTGGTAGATAGAAGTT16810.10450912418742758No Hit
CCTATATTCATTATTGTGAAGAATCTTTACACTGCCTTTCTAGAGAGTGG16800.10444695338184315No Hit
CTGTTACAGTTTAAAATATAATGGCTAAACCTGCTCTCCTTCCAAAAATA16660.10357656210366112No Hit
GAATGAAGTGTTCCCGTTAGTCTGGGTCACAAAAGTAATTCAAGATGCAT16300.10133841310262161No Hit
TCTTTATATTTGATAATTACTATTCATCTATGGTGACTGTAGGGTCTTGT16210.10077887585236175No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)