FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_D04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_D04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915618
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA84380.4404844807263244No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA68260.3563340916612811No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT65160.3401513245333882No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG53860.28116252822848814No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT53540.27949204904109276No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC50920.2658150006942929No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC50190.26200422004804713No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG47750.24926681624415722No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG45040.23511994562590244No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC44100.23021291301292846No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC39990.20875769594981883No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA39730.20740043161006005No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA35230.18390931803731225No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA34960.18249985122294737No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC33550.1751393023034864No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT30460.15900873765019957No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA30170.15749486588662248No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA29540.1542061099864378No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT29190.15237902337522408No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA29060.1517003912053447No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC28640.14950788727188824No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT28060.14648014374473406No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT26910.14047685916503186No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA26820.1400070368935769No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT26470.13817995028236318No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT26350.13755352058708992No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA25470.13295970282175257No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG24170.12617338112295876No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG23690.12366766234186564No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC22960.1198568816956199No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA22780.11891723715270999No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA22550.11771658023676955No Hit
GTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGCCAGTGTAA22210.11594169610016193No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT22070.11521086145567644No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA22010.11489764660803982No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA21930.11448002681119095No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA21870.11416681196355433No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA21680.1131749649460383No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA21350.11145228328403681No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG21300.11119127091100627No Hit
GTTTATTTATTTGACAGAGAGAGAAGGAAAGAGAGCAAACAGGGGGAGCA20450.10675406056948723No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT20440.10670185809488113No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)