FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546080
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA72480.46879850977957155No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA62320.4030839283866294No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT49750.32178153782469215No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT43340.28032184621753076No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG42010.27171944530684056No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC41650.26939097588740557No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG40830.2640872399875815No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG40590.2625349270412915No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA36930.23886215461036944No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC36820.2381506778433199No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC36420.23556348959950327No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC34840.2253440960364276No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA34480.22301562661699265No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA30000.1940391182862465No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC27360.1769636758770568No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA26930.17418244851495396No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA26830.1735356514539998No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT24150.15620149022042845No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT22680.14669357342440237No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT22540.14578805753906654No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA22240.14384766635620408No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG21960.14203663458553242No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC21840.14126047811238746No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT21790.14093707958191037No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA21340.1380264928076167No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT20650.13356359308703303No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA19930.1289066542481631No Hit
GAAGGAAAGAGAGCAAACAGGGGGAGCAACAGGCAGAGGGAGAGGGAGAA19490.12606074717996482No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT18500.11965745627651868No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC18470.11946341715823243No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT18390.11894597950946911No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA18270.11816982303632412No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA18170.11752302597536997No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA18010.11648815067784332No Hit
CTCTGATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATC17700.11448307978888544No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG17260.11163717272068717No Hit
CCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAACTATCCATACAAA16950.10963210183172926No Hit
TCCATGATGTGGCCTGAGGAAAAAGTAAGATACAGTAGATACAGAGAAAT16860.10904998447687053No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA16490.10665683535134017No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT16410.10613939770257683No Hit
CAGTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGA16210.10484580358066853No Hit
CAATAAATATATTTAATAAAAATAGATTAACCAGGGCAACTAGGTGGCTC16020.10361688916485563No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG15740.101805857394184No Hit
GTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGCCAGTGTAA15660.10128841974542067No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC15630.10109438062713443No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA15550.10057694297837111No Hit
GTTTATTTATTTGACAGAGAGAGAAGGAAAGAGAGCAAACAGGGGGAGCA15530.10044758356618026No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)