FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590836
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT30890.1941746352232411No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA23740.14922971318225134No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT22780.14319515022290166No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA21290.13382900562974437No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG18790.11811399792310458No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA18790.11811399792310458No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG18690.117485397614839No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC18680.11742253758401243No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA17740.11151369468631588No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)