FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_B08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_B08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242501
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT28890.23251490340852843No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT22180.17851092272762759No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA20620.1659556008405627No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA19970.16072421672095233No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG18500.14889324032737197No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG17980.14470813303168367No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA17530.1410864055642611No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA17270.13899385191641697No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA15930.12820915234675867No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG15220.12249487123149197No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC14810.11919507509450696No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA14440.11621721028795952No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG14020.11283693131836513No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)