FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652623
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG77680.4700406565804784No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA55660.33679792668987424No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT45310.27417021304919514No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA44920.27181032818737244No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT44010.2663039301764528No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA42850.2592847854592366No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC42460.2569249005974139No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT39060.23635154539178022No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT39030.23617001578702465No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG38150.2308451473808606No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA37650.22781965396826742No Hit
GTAATATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATA37160.22485467042392607No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT33250.20119531193744733No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC30660.18552325606021458No Hit
ATCCAGGTGCCCCCAAATGTTCATTTTTTAATACATCAGGCATTTTTCTC30130.1823162330428658No Hit
TTTTAATACATCAGGCATTTTTCTCAATGACTTCAGATAGGTAAGTCTCC27430.16597856861486254No Hit
GTCTATATGGCACCAACTTTCTTCCACCATTTTCTTTAGTTTCAGTGCCT26350.15944350284366127No Hit
ATATCACACTGTATGTTAATTACACTGGAATTAAAAACAAAGATATGCAT25590.1548447528565196No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT25090.1518192594439264No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC24970.15109314102490407No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA24760.14982243379161492No Hit
AATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGA23980.14510266406796954No Hit
GTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAATA23710.1434688976251692No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG22900.13856759829676824No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG21820.13203253252556693No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT21530.13027774634626288No Hit
CTCATGAGCAAACCGCACATCAGAGGAATTAAAAGATGACCTGATGGAGA21310.12894652924472189No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC21300.12888601937647No Hit
ATTTAAAATGTAGGTAAATCTACAGTCTATCTTGGGCTAGAGATAGAATC20740.12549746675436563No Hit
ATCATGACCTGAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGT20520.12416624965282463No Hit
GCAGAGGGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGT20440.12368217070680972No Hit
AGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGTGAAATTAAACTC20310.1228955424195355No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT19680.11908342071966806No Hit
ATAAAAAAGAGTGCATGTTATAAAAGAAGTCAAAGGCGGTCTTGAATAAG19370.11720761480386029No Hit
CTGTAAGCTTTGAACTACTGACTTGGACATCATTCATGGAGACTTACCTA19120.11569486809756369No Hit
GAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAA18550.11224580560720746No Hit
GCCCAATGTGGGACTCGATCCCAGGACCCTGAGATCATGACCTGAGCCGA18480.11182223652944441No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG18430.1115196871881851No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT18350.11103560824217017No Hit
ATCTACAGTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAA17620.1066183878597841No Hit
TTCCTATACAGATGGCTCTAATTTGGGAAGTACACTGACTTTTTGACTTG17540.1061343089137692No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA17330.10486360168048005No Hit
ATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCATTC17000.10286677602816856No Hit
ATACTGTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAA16950.10256422668690923No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG16690.10099097011236077No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)