FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_B04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_B04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350216
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC53650.3973438323942243No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA30820.2282597747323391No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA29620.21937230783815329No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT27610.20448580079039208No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA25890.19174709824205904No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG25170.18641461810554757No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC24940.1847111869508286No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT22160.1641218886459648No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG21770.1612334619053544No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG21370.15827097294062578No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT21170.1567897284582615No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG20090.14879100825349426No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG19690.14582851928876564No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT19320.14308821699639168No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC19070.14123666139343632No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC18890.13990354135930844No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT18040.13360825230926016No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC17590.13027545222394046No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA17080.1264982787939115No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA16380.12131392310563643No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT16240.12027705196798143No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA16100.1192401808303264No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA16080.11909205638208997No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT15610.11561113184853386No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC15350.11368551402146027No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT15040.1113895850737956No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC14480.10724210052317557No Hit
CAGTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAA14150.10479804712727446No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA13670.10124306036960014No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)