FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436647
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAACTCACTCTTACATGATCTCATTTAATCTTCATAACAATGTCAG26610.1852229531680364No Hit
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG24270.1689350271848269No Hit
ATGTAAGAGTGAGTTAGTACCATGCCTAGCCCAAAGAAGTAGCTATAGAA21680.15090693816922318No Hit
GTCAAAGGATGTGCTGATACTGCCTTCCAGAAAGTTCATGTCAGTTCCAT19930.13872579694246395No Hit
GTGTAGGGGCTCCCAGGCACGGGGTGTCCATCGTAGGTGACGTCGACGGC19160.13336609480268988No Hit
GTATGAAAAGATCTCCAGAAGGAGTGCTCGATCTTGCCCACGCTCTTGAT18890.13148671872770418No Hit
ACATTAGGGATTCATAGGCCATAAGGAGTAGTTTGGATTTTATTCTAAGT17780.12376039486387401No Hit
AGTTAGTACCATGCCTAGCCCAAAGAAGTAGCTATAGAAACGTCAGTTTC17480.12167219922500098No Hit
GTCTTAGGGACAATAACTTTGTGTCACCATTTGAAAATCTCAAATTCTAT17040.11860951228798723No Hit
GAAATAAACAGTTGATTTAACATAAATTCAAGACGTCCAAAAAGATGATG16540.11512918622319887No Hit
GAGTTAGTACCATGCCTAGCCCAAAGAAGTAGCTATAGAAACGTCAGTTT15860.11039594277508671No Hit
CTCTTACATGATCTCATTTAATCTTCATAACAATGTCAGGAATCTAAAAT15840.11025672973249519No Hit
CATTTGGAAAGATGTCACTATCGCCAACTTAACAGGTTACTCTTAACGTT15500.1078901080084391No Hit
GTGTAGACAGTCCCTTCTCTTGTCCCCCGCTCTGAACTACAAGTTTCCTG14900.10371371673069307No Hit
GTTATGAAGATTAAATGAGATCATGTAAGAGTGAGTTAGTACCATGCCTA14630.10183434065570734No Hit
TCCTTCCACAGTGGCTGCACCAGTTTGCATTCCCGTCGGTGCACTAGGGT14490.10085984935756662No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)