FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_B01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_B01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884731
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG129370.6864109520138417No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG57740.3063567161573721No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA39450.2093136898581283No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC39390.20899534204085354No Hit
TTTCAAATACTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACC30030.15933308254599726No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG28990.15381505371323548No Hit
CTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAA20620.10940553320341205No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG20160.10696486660430587No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)