FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581387
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT123960.7838688442487511No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA78410.49583055887015637No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA77220.4883055191423731No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG72300.45719359018380706No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC69290.4381596661664729No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA61800.3907961808210135No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA60390.3818799572780098No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC52720.33337823062918814No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG49220.3112457608415903No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA48260.3051751405569921No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT40070.25338516125401306No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG39940.2525630980904737No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT39270.24832631101684788No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG38030.24048509314924174No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG37750.23871449556623395No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA37390.2364380129595096No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG36490.2307468064426987No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT36460.23055709955880502No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA36070.22809091006818696No Hit
ACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAACACAAAGTAGA34660.2191746865251833No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC31930.20191136009085695No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA31260.1976745730172311No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA31140.1969157454816563No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG30970.19584073980625868No Hit
ATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGTAA30020.189833355149625No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC29460.18629215998360935No Hit
GTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAA27530.1740876837864482No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG24630.1557493516767243No Hit
GGTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATC24460.15467434600132668No Hit
GTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCT24310.15372581158185822No Hit
GTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATA24150.15271404153442517No Hit
CAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTA23500.14860372571672842No Hit
GATGATGAATGGATAAACAAAACATGGTGTATACATACAATGGCATATTA23400.14797136943708278No Hit
CTCTAAGTACCTTATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAA23100.1460743005981458No Hit
CTTCTAATTGTGGTAAAATACAATAACACAAAACTCACACCTTAATCATT22620.14303899045584667No Hit
ACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGT21190.13399629565691384No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA20780.13140363491036666No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA20650.1305815717468273No Hit
CCTCCAAATAGCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAA20140.12735655472063448No Hit
GTTCTAATAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTC19610.12400506643851252No Hit
GGCTACAGCATGGATGAACTTGGAGATCATATGTGAAGTAAAATAAGCTA19420.12280358950718577No Hit
CCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCC19250.12172858383178817No Hit
ACACAAAACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTT19090.12071681378435513No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA18830.11907268745727642No Hit
GTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATC18630.11780797489798513No Hit
GTAAAATACAATAACACAAAACTCACACCTTAATCATTCTAAAGTGTACG18360.11610061294294186No Hit
TTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGAAACTCTGTTGTCCT17680.11180059024135142No Hit
ATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTA17590.11123146958967034No Hit
GCTAAAAGGTTAATCAACCCAAATGTGCTCTGATGATGAATGGATAAACA17370.1098402857744499No Hit
GTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAG17000.10750056753976098No Hit
ACTCACACCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACA16920.10699468251604445No Hit
TCCTAAGAGTAATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCA16350.10339025172206423No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT16220.10256818855852488No Hit
GCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCT15860.10029170595180054No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)