FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353500
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT54270.2305927342256214No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA37630.1598895262375186No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG35950.1527512215848736No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA35580.15117909496494583No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA30620.1301041002761844No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC30150.12810707456978968No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA27320.11608243042277458No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT25510.10839175695772255No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC25260.10732950924155513No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)