FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342617
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC51860.38626056425622496No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA42670.3178121534287142No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT41710.31066193858710267No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA38720.28839199861166664No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG34450.25658843884741517No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA30180.2247848790831637No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG29440.21927325514275478No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC26780.19946120151912275No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT26630.19834398045012092No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG26460.19707779657191887No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT25280.18828899082910466No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC23520.17518026361948344No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC22890.17048793512967586No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT21500.16013501989025908No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG21470.15991157567645872No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC21080.15700680089705404No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA19850.14784558813123921No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC19620.14613251582543643No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA19580.14583459020703596No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC18170.13533271215841897No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA18110.13488582373081826No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG17710.13190656754681343No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT17090.12728872046160594No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA16890.12579909236960354No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA16660.12408602006380076No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA16540.12319224320859931No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA16520.12304328039939907No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC16350.12177709652119705No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA15660.11663787960378871No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA15620.11633995398538824No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA15130.11269036515998235No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA15120.1126158837553822No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT14990.11164762549558063No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT14850.11060488583117896No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC14520.10814699947937498No Hit
AAATTACACTGGCCATTCACCAGAAAAAAAATGAACTCAAGCCCAACCAT14500.10799803667017474No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT14340.1068063341965728No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA14300.10650840857817233No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG14270.10628496436437197No Hit
ATTCTGGACTGAGATTAGTCTAGAGCTGAGAACAGGATTGGGTATTCTAA13880.10338018958496727No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA13870.10330570818036715No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC13850.10315674537116691No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG13590.10122022885156379No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC13570.10107126604236352No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA13540.10084782182856317No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)