FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653982
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC79440.4802954324774998No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT46050.2784189912586715No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA44220.2673547837884572No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT39040.23603642603123856No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA38480.23265065762505277No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG37390.22606050126301255No Hit
CTATTATTCATGCTTGTATTCTGGACTGAGATTAGTCTAGAGCTGAGAAC35110.2122755870378275No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA35060.21197328628727521No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC33650.20344840512170026No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA31660.19141683524971856No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT31070.18784968639320138No Hit
GGATTATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTAT30530.1845848382872365No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC30030.18156183078171345No Hit
GTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGCCCAGG28600.1729160293159176No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG27800.16807921730708072No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG27580.1667490940046506No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT25920.15671270908631413No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA25830.15616856773531998No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA25740.15562442638432583No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA25010.15121083542626218No Hit
ATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGTAAA24690.14927611062272747No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG24280.14679724446819856No Hit
GTCTAGAGCTGAGAACAGGATTGGGTATTCTAAATCCTTTTATCTCTGTT24210.14637402341742534No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC23330.14105353020770478No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG23270.14069076930704202No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT21970.13283094979268215No Hit
GTAGTAGTACTCTTGGATGCCTGCAACACATTTAGGGAACAGAGATAAAA21200.12817551823417667No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC21020.12708723553218837No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT21000.12696631523196744No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC20800.12575711222975824No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA20780.12563619192953732No Hit
GTTAAAAGTGTGATGTTGCTATCTAGTATATAAGCATGCAAATCCATGAA20100.121524901722026No Hit
ATATGGTACATGAATTGTATCAGTAAATTTGTTGAAATATAGTATCTGGC19650.11880419496705526No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA19450.11759499196484606No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA19320.11680901001341006No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC18760.11342324160722426No Hit
ATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCAGT18410.11130713635335815No Hit
ATTCTGGACTGAGATTAGTCTAGAGCTGAGAACAGGATTGGGTATTCTAA18250.11033977395159075No Hit
CATAAAAAGTTATTCTATTATTCATGCTTGTATTCTGGACTGAGATTAGT18190.109977013050928No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA17340.10483790029153885No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA17290.10453559954098654No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC16940.10241949428712042No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC16810.10163351233568442No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)