FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837428
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT100650.5477765659389102No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG91660.498849478727874No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG79050.4302209392694571No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA74500.4054580642071417No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT71490.3890764699351485No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG64880.35310227121824633No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA64180.34929259813173635No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA57540.31315512771112664No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC57410.3124476169950605No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA53280.28997054578465115No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT43560.23707051378339725No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC40960.22292029946207417No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC40900.222593756054659No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG39540.21519210548658235No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC38140.2075727593135622No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT37950.20653870519008094No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA36840.2004976521529007No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA34420.18732706805382307No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA32670.17780288533754793No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT32610.17747634193013279No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT31190.16974814795464094No Hit
CATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGATATGT31100.1692583328435182No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA29400.16000626963342238No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC29260.15924433501612037No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA28540.15532581412713858No Hit
AACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAA27810.15135286933692096No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA27400.14912148938625078No Hit
CTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTA27150.14776089185535435No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG26990.14689010943558062No Hit
TCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTT26990.14689010943558062No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA26560.1445498816824387No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG26440.14389679486760842No Hit
TTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATG26150.14231850173176855No Hit
ATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATA26100.14204638222558927No Hit
ATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATTTT25920.1410667520033438No Hit
GATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCC25800.1404136651885135No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA25090.1365495682007676No Hit
GACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGA24520.1334474058303237No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT24420.13290316681796513No Hit
ACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAAC24150.13143372148459695No Hit
ATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGT23130.12588248355853945No Hit
TCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGAC22950.12490285333629399No Hit
CTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGA22110.12033124563248193No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT21970.11956931101517992No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA21940.11940603931147234No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG21690.1180454417805759No Hit
GGCCACATCATGGATTTTCTTTATTATTATTTTCAGCAATTCAAGTGTGA20410.11107918242238607No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC20290.11042609560755579No Hit
ACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAA20210.1099907043976689No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA19760.10754162884205531No Hit
CCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAA18800.10231693432341295No Hit
CTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCA18790.10226251042217709No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA18590.10117403239745992No Hit
CAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAGACA18490.10062979338510133No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)