FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486952
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCACCTGGGGTCTTAAGGAGAGTGACTTTTATTAACCATTAAGAG70770.28456520270596297No Hit
CTCTCTGGGTGTCCTGTTGCTGGGCTCTGTGGTCCAGCCCCTCCACAGTG58010.23325741711138775No Hit
GAGTGGAGCTGTCCCAGTTTCCCCACAGCTCCCAGGCAGGGGCAGCAACC46860.18842341951111238No Hit
TCCTTAGGGTGATCCCAGGGCTTTTGTCATTCTGTCTTCTCCATTCCCAC43990.17688318873866482No Hit
AGGTTAGACAGGCCAAGGGGAGCTTCGGGCACTGGAGCCGACCCGAAGGG40380.16236742808063848No Hit
GGGTTAGAAAGAGCCCGAGAACCCTGCCCAGGGGTTCAGGGGTCTAGTCA39820.16011567573479504No Hit
TTCCATACCTCTGTGCTTAGAAGTTCAACAGAAAAGCCTCCCCCAGAAAA37350.15018383949509279No Hit
AAGCAGGACTGTAGAGTTGATAGGGGACTAGAATCTCAGGTTGAGGCTTC36370.14624327288986677No Hit
GTCCAGGGTGTATGCGCTGCATGAGAAGTGTGTGTGTGGCCGGGGGAGGC36320.14604222357327362No Hit
TCCCAAGAGTGACAAAATTCTTAAGAGAAGCTTATCTTCGGAGGCACTCG34680.13944780598901788No Hit
GTCTGGACCCTTGAGGACATGTGGTGAGTGAGGCTGAAGCGGGCAGCCCT33570.13498451116064966No Hit
GTCTTAAGGAGAGTGACTTTTATTAACCATTAAGAGTTGGTTTCAGCCAG30870.12412784806461886No Hit
GTGCTGAGGTGATGGCCTTGTTCCTGGTGGCTCCTCAGCCTCTCAGAACG30450.1224390338052363No Hit
GATAAGAAGAGGATAGCGGGATGGGAGGAGAGCAAAAATGGTTGGGAAGA29760.11966455323625064No Hit
GCCTATAGGTGGGGAGGGTCTCAGGGTCTCCAGTAGAGAAAGGAACCCTT29740.11958413350961337No Hit
CCTATAGGCTGCACACAGCCTCTTCAGGGATCCTGCCCTCTGGGCACTAA29680.11934287432970159No Hit
ATATAGGAATCATCAAGATCTATTGTGAAAGCATAGAAGGAGGATGGGGG29640.11918203487642703No Hit
TTAATATGCTGTCCGTGAGCAACATGTTGGTATCATTTTATCAGACTGGC29260.117654060070319No Hit
GTTTCATTCCATACCTCTGTGCTTAGAAGTTCAACAGAAAAGCCTCCCCC29210.11745301075372584No Hit
GTAGAGTTGATAGGGGACTAGAATCTCAGGTTGAGGCTTCTGGGTAAGGT28970.11648797403407866No Hit
GAATACAATAGTAGGACAAAGTACAGGGAAAACCCTGAATTTGATTTTAG28810.11584461622098054No Hit
GTTAATATGCTGTCCGTGAGCAACATGTTGGTATCATTTTATCAGACTGG28630.11512083868124516No Hit
GTACAGGGAAAACCCTGAATTTGATTTTAGATACCTGAGTATGGCTATGT28530.11471874004805882No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT28230.11351244414849984No Hit
CCTCTAGACCTTGCTTCCTTTTATCCAATAAAATTTTACTCCACAGACTT27340.10993376631314156No Hit
GCATGAGAAGTGTGTGTGTGGCCGGGGGAGGCAGTGGGTAGTGAAATATT27050.1087676802769012No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)