FastQCFastQC Report
Wed 5 May 2021
HK3CVBGXH_n01_S10_R2_A01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3CVBGXH_n01_S10_R2_A01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722889
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATATAATATCCAGTGTTCATTACATCATGTGCCCTCCTTAATGTC92500.339712709552244No Hit
CTTCTTATACAGACATCAATCACTGAATTAGTGACTGATCTTCTAATCCA50730.1863094676279496No Hit
ATAATATCCAGTGTTCATTACATCATGTGCCCTCCTTAATGTCCATCACC50370.18498734248807058No Hit
ATATAATATCCAGTGTTCATTACATCATGTGCCCTCCTTAATGTCCATCA47560.17466742125734835No Hit
CCTTAATGTCCATCACCCAGTTAAGTTGGTGACTTTTTATTCCCTTGTTC42810.15722271455061151No Hit
CTTATACAGACATCAATCACTGAATTAGTGACTGATCTTCTAATCCACTA32160.11810984582919098No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)