FastQCFastQC Report
Tue 16 May 2017
HK2HHAFXX_merged_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK2HHAFXX_merged_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134017329
Sequences flagged as poor quality0
Sequence length75
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCATCTTCCCGGTGGAATCGAAGAGGCGG5179082738.64487330589912No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2956100122.057595999394973No Hit
GGATACCGTGTTACGACTTCTTCATCCTTTAAGACATGCAACTAAATCAAACCTGACTTACAGATTTGTTATTGT10377520.774341652488836No Hit
TGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCATCTTCCCGGTGGAATCGAAGAGGCGG10247720.7646563378382208No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTA7315460.5458592597379701No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATAAAGGCGGTTTCCACCAAAGAATCTCCACCCC7090860.5291002330004652No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAACCCTAATTAGAGGCAGCCTCACTGGA5901070.4403214154491917No Hit
AGATACCTTGTTACGACTTAACCCTCCTTGCTAAATCTCAGTTCGACAACCCCAAAAAGAGGCAGCCTCACTGAA4531630.3381376150243973No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAGATTCTCCGCTCCAAAGAAAGGTCGC3700480.2761195158575351No Hit
GTAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCATCTTCCCGGTGGAATCGAAGAGGCGG3531020.2634748824161389No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATAAAGGCGGTTTCCACCAGGAATCTCCACCCCG2689320.20066957161935378No Hit
GCAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCATCTTCCCGGTGGAATCGAAGAGGCGG2493440.1860535513284256No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAGCTTCTCGTCGACGCAGAAAAGTTGC2181380.16276850287025196No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATTTGGTTCGCATAAGTTTCCCGCTTGTGGCAGGTCCAAA2024710.151078223622857No Hit
GGAAACCTTCTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCATCTTCCCGGTGGAATCGAAGAGGCGG1852740.13824630096903365No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAACCCTAATTAGAGGCAGCCTCACTGAA1647560.12293634056831562No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGGGCATCTTCCCGGTGGAATCGAAGAGGCGG1463300.10918737232854417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTA69516250.068.96461525
TAAATGA69939750.068.9570130
ATGATCA68243500.068.94933
AATGATC68328100.068.9478432
TACGACT72931350.068.94572412
CTTTTAC65539350.068.9446617
TGTTACG71429850.068.939839
ACCTTGT70027600.068.938715
CTTGTTA70145050.068.9384847
GCATCTT68079200.068.9360947
TCGAAGA67288550.068.9357564
CGACTTT66762100.068.9352414
ATCTTCC68088050.068.9337549
GAATCGA67619850.068.9331461
CGAAGAG67230850.068.9322465
TTGGGCA65538000.068.93062643
TGGAATC67685650.068.9303459
GGAATCG67708650.068.9287360
TCTTCCC68254350.068.9284150
TGATCAA68136300.068.92734
Produced by FastQC (version 0.11.2)