FastQCFastQC Report
Tue 16 May 2017
HK2HHAFXX_merged_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK2HHAFXX_merged_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134017329
Sequences flagged as poor quality0
Sequence length75
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCGG4992271437.25093939157674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3544262226.446297851526353No Hit
GCTTCAACTGATGAGATCGTACAACAATAACAAATCTGTAAGTCAGGTTTGATTTAGTTGCATGTCTTAAAGGAT10491660.7828584615352243No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATG7723800.5763284537628712No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCGC7324310.5465196221005121No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCGAAACCTTTCGGGGTGGAGATTCTTTGG7181060.5358307058932655No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGCGGCGAGGGGGCCCGCCTCTTCGATTCCACCGG6902180.5150214566655033No Hit
AAACCATCCGAGTTGGGTTCCAGTGAGGCTGCCTCTAATTAGGGTTGTCGAACTGAGATTTAGCAAGGAAGGTTA6076960.4534458375901522No Hit
AAACCACCCGAGTTGGGTTTCAGTGAGGCTGCCTCTTTTTGGGGTTGTCGAACTGAGATTTAGCAAGGAGGGTTA4685000.349581657458641No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCGAAGCCTTTCGGGGTGGAGATTCCTGGT2721920.20310209286442354No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGGAGAGGGCGACCTTTCTTTGGAGCGGA2679270.19991966859748417No Hit
CCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCGG2295960.1713181434917271No Hit
GCTACTACCGATCGAATGGCTTAGTGAGGCTTCCGGATTGTTGATGCGGAGAGGGCAACTTTTCTGCGTCGACGA1982910.1479592239896081No Hit
GCTCCTACCGATTCCGAGTGATTCGGTGAGCCTTTTGGACCTGCCACAAGCGGGAAACTTATGCGAACCAAATCA1737320.1296339818860291No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGCTGCCTCTAATTAGGGTTGTCGAACTGAGATTTAGCAAGGAAGGTTA1673570.12487713435924395No Hit
GCTACTACTGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCGG1536200.11462696738270318No Hit
TCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCGG1522170.11358008784072991No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCCG1476090.11014172652254546No Hit
GCTACTACCGAATGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGGGGGCCCGCCTCTTCGATTCCACCGG1466690.10944032469114497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC71238400.069.0099642
TACTACC71443350.069.007893
ACTACCG71246850.069.006954
AGGTCTT65940300.068.9863727
GAGGTCT65957300.068.9860826
CCGATTG71707300.068.985088
CGATTGA71668850.068.981199
TGAGGTC65867050.068.9800225
GGTCTTC66026000.068.971628
GCTACTA70802100.068.967841
GAATGAT70837550.068.96580514
GTCTTCG68413650.068.96400529
CTTCGGA68047950.068.963631
TCGGACC68656150.068.9570533
ACCGATT72043850.068.95617
CTACCGA71921200.068.952085
GGACCGG67188250.068.94762435
GACCGGG67170450.068.94594636
ACCGGGG59450050.068.94546537
TTTAGTG69929950.068.9432320
Produced by FastQC (version 0.11.2)