FastQCFastQC Report
Wed 17 May 2017
HK2HHAFXX_merged_I1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK2HHAFXX_merged_I1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134017329
Sequences flagged as poor quality0
Sequence length12
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGG5093030838.0027779840322No Hit
CTTATCAAGGCG43035803.2112115889132515No Hit
TACACGATCTAC37811422.8213828974311226No Hit
GACTTTCCCTCG35378662.6398571187760353No Hit
TCCGACACAATT35164822.6239009732838356No Hit
GCTGATGAGCTG32492022.42446407807456No Hit
TGCCTACAAGCA28367392.116695670005481No Hit
CACGCCATAATG28144062.1000314071324313No Hit
ATCCCGAATTTG27804402.07468692350972No Hit
AACGGATAAACG25544791.906081115823462No Hit
CTAACCTCCGCT25523541.9044954999812003No Hit
GGAGACAAGGGA24947501.8615129988152501No Hit
CCTCGTTCGACT24447191.8241812594250406No Hit
TGAGAACCTCCA22236891.6592548266649905No Hit
GGATCGCAGATC22105061.6494180390656792No Hit
CAGGGTGATACC19477341.4533448879584818No Hit
TAATGACCACGC18288201.3646145715976774No Hit
TTACTGTGCGAT17915001.3367674265467566No Hit
AATCAGTCTCGT17668611.3183824906702923No Hit
ACGCGCAGATAC14007561.045205131643834No Hit
AATCCGTACAGC13723051.0239757874893924No Hit
GTATGCGCTGTA13634931.0174005184060937No Hit
ATGTGCACGACT12574580.9382801533076367No Hit
ACCGTGCATAGG11324070.8449705783943806No Hit
CAGGCGTATTGG10520550.7850141529085392No Hit
TTCAGACTACCA10010150.7469295258078155No Hit
CCAGTGTATGCA9914220.7397714962667253No Hit
ACACCTGGTGAT9616510.717557204859679No Hit
GACTTGGTATTC9023120.6732800949942824No Hit
GCACACACGTTA8820390.6581529467730252No Hit
TATCGTTGACCA8710420.6499472915178006No Hit
AGCTGTTGTTTG8675790.6473632973240349No Hit
TAGGATTGCTCG8247680.6154189209367096No Hit
AGGCTACACGAC6397220.4773427472203986No Hit
GCGGATGTGACT6057470.4519915480482379No Hit
GGATGGTGTTGC5247510.391554587690671No Hit
ATTTCGACATGC4914940.36673914013015435No Hit
TGCCCATTTAAG4359680.3253071847148961No Hit
AGCAGTCGCGAT1801310.13440873754467977No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTGT852.4450026E-56.0001165
TCTACGC806.53934E-56.0001165
AGTCTGT909.1527E-66.0001165
CGTCTTG1950.06.00011545
TCTAGGC9600.06.00011545
ATTCCTC7550.06.00011545
TGTCTGC2000.06.00011545
CTTCTTT600.00339637116.00011545
TCGAGGC2150.06.00011545
ATTGCTT650.00126191126.00011545
TGTCGAT1451.9645086E-106.00011545
TCTATGC5750.06.00011545
TGAAGGC7150.06.0001155
ATGTAGG1259.680662E-96.00004434
TCGTTAC2350.06.0000443
ACTTCCG806.540473E-56.0000444
TAGCAAG6800.06.0000443
TCTACAG600.00339682456.0000443
TCTAACG1054.832382E-76.0000443
TCGTCTC806.540473E-56.0000443
Produced by FastQC (version 0.11.2)