FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13133806
Sequences flagged as poor quality0
Sequence length110
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG799730.6089095575189705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG183750.083.829572
GTATCAA209900.073.133379
AAGCAGT261550.059.6202961
GGTATCA264350.058.170988
GCAGTGG276100.056.1137543
CAGTGGT296500.052.270124
GTGGTAT298400.051.8975146
AGTGGTA301400.051.545365
TGGTATC387250.040.1198437
ATCAACG209750.039.52597410-11
TACATGG211200.038.4957722-23
CATGGGG105400.037.7571324-25
CAGAGTA204250.037.632218-19
GAGTACA209000.036.5411220-21
CGCAGAG220850.035.075616-17
GGGTCCG19200.034.23944528-29
AGAGTAC243350.033.8593418-19
AGTACAT244650.033.4673920-21
GCAGAGT253400.032.8663716-17
ACATGGG232550.032.08107424-25
Produced by FastQC (version 0.11.5)