FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n02_Nikki_DEPDC_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n02_Nikki_DEPDC_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33756152
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT676470.20039902652411332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG612880.18156097886986644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1466650.090.7090761
AGCAGTG1582450.083.9758152
GCAGTGG1678050.079.0254753
GTGGTAT1749050.075.846436
CAGTGGT1779850.074.518314
GGTATCA1785000.074.299768
AGTGGTA1812950.073.232865
GTATCAA1837050.072.239349
TGGTATC2064550.064.4374857
CAACGCA1746200.041.3341212-13
GCAGAGT1744500.041.2813916-17
ATCAACG1840250.039.28295510-11
CATGGGG929050.039.10197424-25
TACATGG1843050.038.63984722-23
GGGTCCG145200.038.10278328-29
ACGCAGA1929850.037.46155514-15
CAGAGTA1785100.037.2049318-19
TCAACGC1796900.037.03228812-13
AACGCAG1798050.037.0293514-15
GAGTACA1836250.036.0754620-21
Produced by FastQC (version 0.11.5)