FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n02_Nikki_DEPDC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n02_Nikki_DEPDC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28371599
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT569610.20076767615388896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG512650.18069126100365368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1252450.090.6106951
AGCAGTG1345250.084.192782
GCAGTGG1421300.079.611363
GTGGTAT1485000.076.2314766
CAGTGGT1505050.075.260564
GGTATCA1522000.074.393428
AGTGGTA1534150.073.857825
GTATCAA1565000.072.437859
TGGTATC1752550.064.851987
GCAGAGT1475500.041.5214216-17
CAACGCA1486800.041.32453512-13
ATCAACG1559550.039.43955210-11
CATGGGG796850.039.09602424-25
TACATGG1566450.038.81040222-23
GGGTCCG122000.037.82484428-29
ACGCAGA1633400.037.6675314-15
CAGAGTA1512500.037.508818-19
TCAACGC1520200.037.3260512-13
AACGCAG1526300.037.17130714-15
GAGTACA1560400.036.2933420-21
Produced by FastQC (version 0.11.5)