FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n02_Nikki_DEPDC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n02_Nikki_DEPDC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36612950
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT726490.19842432800416246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG662110.18084038571052047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1612550.090.924911
AGCAGTG1731750.084.542652
GCAGTGG1829800.079.855073
GTGGTAT1903100.076.7460566
CAGTGGT1938150.075.381554
GGTATCA1946100.075.036788
AGTGGTA1975400.074.002065
GTATCAA1997500.073.184359
TGGTATC2257600.064.921537
GCAGAGT1893800.041.57957516-17
CAACGCA1903700.041.49267612-13
ATCAACG2004200.039.4570810-11
CATGGGG1012550.039.23031224-25
TACATGG2012850.038.7341422-23
ACGCAGA2089150.037.85186814-15
CAGAGTA1940700.037.7406518-19
GGGTCCG156600.037.61941528-29
TCAACGC1955650.037.51473212-13
AACGCAG1966700.037.31086314-15
GAGTACA1998400.036.56167220-21
Produced by FastQC (version 0.11.5)