FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n02_Nikki_DEPDC_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n02_Nikki_DEPDC_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32089953
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG667610.2080433087577286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT640610.19962946034853962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1407650.090.778831
AGCAGTG1515400.084.364622
GCAGTGG1607500.079.54233
GTGGTAT1676100.076.157746
CAGTGGT1703650.074.9942864
GGTATCA1712150.074.4728558
AGTGGTA1728750.073.968155
GTATCAA1750150.072.665829
TGGTATC1979850.064.6545947
GCAGAGT1669700.041.3706416-17
CAACGCA1666500.041.35378612-13
ATCAACG1755200.039.3061110-11
CATGGGG896350.038.97034524-25
TACATGG1765400.038.64253622-23
ACGCAGA1844000.037.48634314-15
CAGAGTA1708400.037.4551118-19
TCAACGC1707100.037.33937512-13
AACGCAG1717900.037.11887714-15
GGGTCCG142250.036.73547428-29
GAGTACA1755200.036.3531920-21
Produced by FastQC (version 0.11.5)