FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13133806
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG177230.1349418439711992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC23350.020.8220214
CCGGGAG18450.020.628483
CCGATGG19450.020.2524851
ACCGGGA19450.019.5681152
CGGCCCA6450.019.4370389
TACCGGG25050.019.4146421
GCCGTGA10600.019.2035161
CCAAGGT25600.019.1638345
ATTCCAG20650.019.0702082
GGCTAGC15700.019.0570964
TTGGATC21600.019.046073
CACTACG21200.019.0377319
GCACGGC13300.018.9398382
TCCATAC28600.018.8799329
TCGGAGC14100.018.874758
TCGGTGA17250.018.8706828
GGCAAAC13700.018.8683876
GGATCGG17200.018.8021015
TAGCACA13850.018.7489551
AGGAACG8750.018.7267848
Produced by FastQC (version 0.11.5)