FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n01_Nikki_DEPDC_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n01_Nikki_DEPDC_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33756152
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC197650.020.8008984
CCGGGAG153800.020.3794233
GGGAGCA179050.020.166045
CCGATGG163450.019.856381
ACCGGGA158350.019.8080922
AAAGAGA104400.019.7902324
GGAGCAG150300.019.6316226
ACTGTAG192950.019.5172163
CGGAGCA120400.019.4677729
AGAGAGG185100.019.4668988
CCATACA254800.019.3909710
GCGACGT104600.019.342542
GGATCGG144100.019.262375
GAGAGAG187300.019.255867
AGAGGGC185700.019.22038510
TGAATAC187200.019.1780110
TGACGCG155400.019.1569561
TGCGACG111950.019.1404461
CCAAGGT215800.019.1177275
AGCCAAT184550.019.1008681
Produced by FastQC (version 0.11.5)