FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n01_Nikki_DEPDC_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n01_Nikki_DEPDC_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28371599
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC162000.020.7402734
GGGAGCA143750.020.0978135
CCGGGAG125650.020.0421243
AAAGAGA85050.019.7914474
CCGATGG131000.019.7187021
GGAGCAG121050.019.694976
CCATACA208300.019.4107610
ACCGGGA129900.019.3699022
ACTGTAG168100.019.3658983
CGGAGCA106900.019.3641419
GGATCGG123050.019.2944225
GCGACGT83400.019.2864252
AGAGAGG151450.019.2384038
AGCCAAT154500.019.2056031
GCCGTGA74250.019.195961
TATTCCA172650.019.1564241
CCAAGGT178450.019.1490335
TGAATAC164950.019.10419310
GAGAGAG153650.019.0560047
TGACGCG128700.019.0363251
Produced by FastQC (version 0.11.5)