FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n01_Nikki_DEPDC_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n01_Nikki_DEPDC_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36612950
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC205300.020.8241314
CCGGGAG160450.020.2751773
GGGAGCA184100.020.2043825
GGAGCAG153550.019.9253236
CCGATGG173250.019.7628171
AAAGAGA114050.019.7361934
ACCGGGA166000.019.6045442
ACTGTAG204350.019.558873
CGGAGCA131750.019.5104569
GGATCGG157300.019.4642965
GCGACGT103850.019.4505922
AGAGGGC200000.019.3915110
CCATACA265200.019.33976610
AGAGAGG203200.019.210638
CCAAGGT239900.019.1782075
AGCCAAT207800.019.1727811
AAGGTTC236450.019.0862887
TATTCCA232250.019.0025021
TGAATAC202150.019.0002710
GAGAGAG206800.018.9453537
Produced by FastQC (version 0.11.5)