FastQCFastQC Report
Wed 9 Dec 2020
HJYYHAFX2_n01_Nikki_DEPDC_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYYHAFX2_n01_Nikki_DEPDC_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32089953
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC184550.020.8464184
CCGGGAG138600.020.2486953
GGGAGCA162750.020.245435
GGAGCAG135100.019.788016
ACCGGGA141950.019.7790432
CCGATGG154200.019.7695851
GGATCGG136350.019.6227385
CCATACA233950.019.55364610
CGGAGCA119050.019.5315849
AAAGAGA100100.019.3651854
GGCAAAC110600.019.3371436
ACTGTAG179050.019.3178163
GCGACGT98450.019.299082
AGAGGGC179000.019.2578510
GAGAGAG180650.019.2341737
AGAGAGG180600.019.2212288
AGCCAAT173350.019.1727871
CCAAGGT213550.019.1540685
AAGGTTC208050.019.106557
TATTCCA201650.019.0853251
Produced by FastQC (version 0.11.5)