FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_pIXHALO_ampPN94-B_w1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_pIXHALO_ampPN94-B_w1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16670603
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCC2196281.3174568430428102TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATGCC441130.2646155031104754TruSeq Adapter, Index 3 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)