FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_pIXHALO_ColYi5UMI1-B_wmh6498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_pIXHALO_ColYi5UMI1-B_wmh6498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGAGGCAGCACCGACGACGGCGTTAGGTCTGATATGTCCTCGGAAAAT3810.326086956521738No Hit
CTAGAGTTTGTTCTTTGAGATTAGATCTTCAAGGAATCCGTAACGAAGAC369.782608695652174No Hit
TCTACTCCGAGGGAAGATCAGCCTCAGATCCCGATTTCTCCGGTCCACGC338.967391304347826No Hit
CAACAATAAGGCTGATGAGATAGCATCAGAAGCCCACACATAAACCCATC297.880434782608696No Hit
CGTCTTTCAGCCATAACTCGTTTCTTTCCAGTAATATCCATCAACTTGCA246.521739130434782No Hit
ATTTCTAGAATTTTGGGGGCCACAATCAATTGTTTCTCCTTACTCTGCTC236.25No Hit
AACGGTTGTAGTTCTTTATTCAGTTTAGTTAGTTAAAGCAATTATACAAG215.706521739130435No Hit
AATTGTATACTTTGTTATGTTTGCTATGTTTGTCAAAATTATTTGTTATA205.434782608695652No Hit
CCAAGTCAAGATGATACGGATTAATCCTTTATTCTTGCGCCAAAGATCTT154.076086956521739No Hit
AATGAGATTATGCAAGAATCTTACTAACCAAAAAAAATTGCATAATATTT154.076086956521739No Hit
CCTGTGAAGCAGATAAAGAAGAAGACAACATTAAGTTCCAAACTTAAACC92.4456521739130435No Hit
CTTATTAATTTATCGAGTTATATCAATTTTTATATTGATCTTAATTCGAG92.4456521739130435No Hit
GCTCTCTTACCCGTCACGTCCCTACAAGATCCATTATCTGAGTCAAGATC10.2717391304347826No Hit
TACTAAGTTCAGAAGAGAATGGTGAGCACGTAATTCTTCATTATTTCATT10.2717391304347826No Hit
AACAGTAAATTTATATTAATTAATCATAAGTTAATGTTTAAAGACAAATT10.2717391304347826No Hit
ACTATCTGATTTGACAAGCTGAGAGAAATCCATGAGAAAGAACATTGAGC10.2717391304347826No Hit
AGCCAAGCAAATGATTAACACATCATATAGAAATGTAAGGGTCATGTTAA10.2717391304347826No Hit
CTACTTAGGCTTTTGAAATCGAGTTGCGGTTTAAGTTGTTATACTCAATT10.2717391304347826No Hit
AGACCACCCCCGAGCCCTTGTTCATTACCAAGGAGAAAACTGAGATTTCC10.2717391304347826No Hit
AACGGTTGTAGTTCATTATTCAGTTTAGTTAGTTAAAGCAATTATACAAG10.2717391304347826No Hit
AGGTGTTTGACACCTTGATACCTTATTCAACAAATTCAGTATGCCAAGTA10.2717391304347826No Hit
AAGGAGGTGATCAGTACCGGGCAAATCTTGCCCTCAAGATGAACGCAAAG10.2717391304347826No Hit
CGTCTTTCAGCCATAACTCGTTTCTTTCCAGTAATATCCATCAACATGCA10.2717391304347826No Hit
GACTGTCCAACGATATAATAGGTTTCTAGGAACTAATTCGAAGGGTTGAT10.2717391304347826No Hit
AGAAAAAAATCAAAGGAAGATGCTTAGGAAAGAGAGAGAGATGCTTATGC10.2717391304347826No Hit
GATGTATTTTGACTATATGTTTTTTTTTTTTTTTGTGTTAAGAATACTGT10.2717391304347826No Hit
CGCACGGACACACATGAGATGTTCGGATTCAGAAGACACCACATGTCGTA10.2717391304347826No Hit
AATGCTCTAGGAAATTAACAAATCTGATTAGGCAAAAGAATTAACAAATT10.2717391304347826No Hit
TTATCATTGGGGGTTTTCTAAAAAATGGAGTTGATTGGATTTGCACCAAT10.2717391304347826No Hit
TGTCTCATAAACCAAACGAAATTACGTTTTAACATTTAAAACAACAGAAG10.2717391304347826No Hit
ATATATACATTAATATACAACATATGAATATGCCTGTGTATACTTACTTG10.2717391304347826No Hit
CACCATCACCACCAAAAATACCCGTTAATATAATCTACGGAGGTTATAAA10.2717391304347826No Hit
AACGGTTGTAGTTCTTTATTCAGTTTAGTTAGTTATAGCAATTATACAAG10.2717391304347826No Hit
GCAGGCTCATCAACAAGTTAATTTGTTAGATGTTTAACTATGCAATTTAA10.2717391304347826No Hit
TAAATAACAACTTAAATTAACCTCACATAATCAAACATTAAAAAAAAAAG10.2717391304347826No Hit
CCCGAGGCTCTTGATTTTTTTGTTCTATGAATGGGATTCATTTAATTATG10.2717391304347826No Hit
CGTGTATCTCCGTCACTTGTAGTGATTTATCATTCAATGAATGACTGAAA10.2717391304347826No Hit
AACCCCTTTTAAAAGCTTTTAAAATCCCTTGTAAATTATCTATGACACTT10.2717391304347826No Hit
GTATGGCAGCTCAAAACTAAGATTTCGTAATGGGTAAAGAGAAGACTCAC10.2717391304347826No Hit
CAACAATAAGGCTGATGAGATAGCATCAGAAGCCCACACATAAGCCCATC10.2717391304347826No Hit
GGAAGATGAGAGAGTAATCCTACAATTCCTAATCCATAGGTAAATTGAGA10.2717391304347826No Hit
AAAAGCGCTTTTGAAACGCAGAAACAAACAGGCCCTACTGAAACAAACCC10.2717391304347826No Hit
TCTACTACGAGGGAAGATCAGACTCAGATCACGATTTCTACGGTCCACGC10.2717391304347826No Hit
GGTCGACTATGTTGAAGGTGACTATAATGGTAGGAAAAACCAATTCCAAA10.2717391304347826No Hit
ACAAACCAAGCTTCTTCTTGCTTCTCAAAGTTTTGATGGTTTAGCCACAG10.2717391304347826No Hit
CTAGAGTTCGTTCTTTGAGATTAGATCTTCAAGGAATCCGTAACGAAGAC10.2717391304347826No Hit
ACTAGTTTTGCCTAATAAAACCAGGAATCGTATTCAAAACCGAACGACAA10.2717391304347826No Hit
AAACGTCACACATCCGCCGCCTACTTACCGTTTAGGTGGCACCAGCGAGA10.2717391304347826No Hit
CATGGTGGGTTTTGCGCCCCTCACTTCCCGTGGCTCGCAACAATACATCT10.2717391304347826No Hit
ATTTTGCAAGTTTGTTATTACGGGTAGTTCCTGCAAAGGATCGGACTAAT10.2717391304347826No Hit
GTTGGGTATAAGATGTCGGATCACTATAAAGATTTTATTAATGAAGGAGA10.2717391304347826No Hit
ATTATAATATCTTAATTAGAATTAATAGATGCCCCAAACAAATCGGAAAA10.2717391304347826No Hit
CATAGATGTAGGGCACGTCATTTTGGGTAGGCTTTGGTTATATGATTTGG10.2717391304347826No Hit
CTAGAGTTTGTTCTTTGAGATTAGATCTTCAAGGAATCCGAACGAAGACA10.2717391304347826No Hit
AAGATAAGTTTTGAATTTTCATCCTACTCAAGGACCTGGATTCGTTCGTA10.2717391304347826No Hit
CCGAGCTCCTCGTTGTTTGCACAATCAGCACGATCATCGAGGAGAGTAAC10.2717391304347826No Hit
GGGAAGTCAGCCATTGGAACTTAGGAGGAGAGAGAAAAGAAAACCAATGT10.2717391304347826No Hit
ATAATAGTGTGAAGTCACTCATAGTGGAGAACTGATGAAGCATCATGAGT10.2717391304347826No Hit
GCAACATCGCTGGCATTCAGGTCCAATGCAGCTGTTCCGCTTGTGCTTTG10.2717391304347826No Hit
AATTATTTATTTCACTTGTATTGGAATATGTAATATCATAAATAATAGTA10.2717391304347826No Hit
AAACTCTAAAGGCTTATGTGAAGAATTTTGCAACGCTTGAAGCTTGTATG10.2717391304347826No Hit
CAACAATAAGGCTGATGAGATAGCATCAGAAGCACACACATAAACCCATC10.2717391304347826No Hit
TAAACATAAAAGAGGGAAGCAACACGAGCACTTCACGGGAGGTCACCCAT10.2717391304347826No Hit
AGAGTATATAACTTCGATCATAGGGGTCAATTTCTAGTCGCATAGCTTCA10.2717391304347826No Hit
GGTGCTATCAAAATGAACTCCCATTGCTGATATGGACCAAGAGGAAATTA10.2717391304347826No Hit
GTTGTTCTTGTGTAGAGCTTCAATCTCCTCTTGCATTGCTATCATCCACA10.2717391304347826No Hit
CAACAATAAGGCTGATGAGATAGCATCAGAAGCCAACACATAAACCCATC10.2717391304347826No Hit
TAGACTCAAGGTCCAACTTTCCAGTTCAGATGTTGACACCAAACCAAATA10.2717391304347826No Hit
GCCAGCACGAAGGCCGTGCGATCCGTCGAGTTATCATGAATCATCAGAGC10.2717391304347826No Hit
AAAACATTTAATCTGCCATATCTTTCAGGTTTCAAAAACATGAACTCATG10.2717391304347826No Hit
GTGAAAGTAGGAGATGTGAGCAAGATTTTATGACAGCATTTGAGCAAGAA10.2717391304347826No Hit
ATTCGAAGCATTTAACCACTCTCTACATTATGGGCTTGCGCGTGTTGGCA10.2717391304347826No Hit
GTACGTACAATTACAGCTCTAATCACTTCTGATCTTTCTAGAGGAGTATT10.2717391304347826No Hit
AAGGAAACAAACTGGAAGTTTTGCAGAGGCCTTTTCTGGACATGAAGAAA10.2717391304347826No Hit
AAAAAAAAGAGAGAGATGCAATCAATGAAACAAGCAGCAGAAATCAATTT10.2717391304347826No Hit
ATCATACCTTCTACAACGAGCTTCGTGTTGCCCCAGAAGAGCACCAGATC10.2717391304347826No Hit
ATATGAGTCACACAACTCACCAGAAACAATATCTCTATTAGCAGAACCAA10.2717391304347826No Hit
CCTGTTTAATGAGACACTTGTAGAGAAGTTTGATACATTCTATGTTTCAT10.2717391304347826No Hit
AGAGCTGCCCTTGCCACAGCGAGTAGCAAGGACAATAGGGGAAAGGCAGG10.2717391304347826No Hit
AAATGACACGTGGCAAATTTATCTCACTTTCAAAACGTTGGCGTATACTA10.2717391304347826No Hit
GCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCC10.2717391304347826No Hit
AATATCATCAACATCAGCTTTGTTCTCACAGAATATTAAAACCGGTGGAG10.2717391304347826No Hit
CGAAGTCGATGCTCTCGAATTCAACGTCGTCGACGTCATCATCTTTGACG10.2717391304347826No Hit
TATCAAAATTCAGAAGCACAAAAACAATGCAAAATGTCAAAAGTGTAAAC10.2717391304347826No Hit
AGTTGTTTAACTGACATTTATTTGGATAAGCTATATAAAAATAAGTGATC10.2717391304347826No Hit
GAGTACCCAAAAAAAGCTGTTCCAGAATTACCTGTCCCTTTTTGGTTGTA10.2717391304347826No Hit
AAACAAGATTTCATGAGCCATGAGAAATGGCGAGAATTGCAGTTTTCTGA10.2717391304347826No Hit
AGTCTACTTCCCTTCGAATGCCGGAATACTCTTTACCTTAAGTGAAAGGA10.2717391304347826No Hit
GGGATTGCTAAATCACCGATGAAATAAAGCAACGGAGCCACCATAGTATT10.2717391304347826No Hit
GGCGCGCGCGCGCGTGGTATTTTTGGGCAAAAGGTAGATCGGAAGAGCAC10.2717391304347826No Hit
ATGTAATCACTAGAAGAGCTATTGATAATAATGATCCGCATAACAGAGCG10.2717391304347826No Hit
AAGGAGGCAGCACCGACGACGGCGTTAGGTATGATATGTCCTCGGAAAAT10.2717391304347826No Hit
CACAGAGGAGCCAAGCTTTATCTCGAAACAGGGGTGATTTATGATGATGT10.2717391304347826No Hit
GATTTGGAAATCTCAGTATAGTTAATGAAGGGGTCTGCCTCGACAAATGG10.2717391304347826No Hit
AACATGAGTCGATTCAATATGTCTCATGTTAGATCGGAAGAGCACACGTC10.2717391304347826No Hit
GATAACATGATAACATGCAATGTTGGGGTAACTTGAACCAAAATAACTTG10.2717391304347826No Hit
ATTTCTTACCATAAAAAAAGAATAAACAAAAGAAAGAAAATGGGGATGCA10.2717391304347826No Hit
CGTCTTTCAGCCATAACTCGTTTCTTTCCAGTAATATCCATAAACTTGCA10.2717391304347826No Hit
ATCCCAAAATACATTAGTTCCTCAACTTTTTTTTTTTGCAGATCGGAAGA10.2717391304347826No Hit
CTAGAGTTTGTTCTTTGAGATTAGATCTTCAAGGAATCAGTAACGAAGAC10.2717391304347826No Hit
CGTCTTTCAGCCATAACTCGTTTCTTTTCAGTAATATCCATCAACTTGCA10.2717391304347826No Hit
AAAAAATAAAACCTAAAACAAAAAACGTCATTGTAATAAAATTGGTTCAT10.2717391304347826No Hit
AATCGGCAGCAGGTGCTGGCGATGAGTCTGGACAGTCAACTGCACGACGA10.2717391304347826No Hit
CCTCGTGCACATTTGACACTGTGACAAACCACCCCCACCAAAAGAACCCG10.2717391304347826No Hit
AGGTAATAGAATCAATGGATATATGTTTATGTCTCTTACATTGTCATTTT10.2717391304347826No Hit
AGAATACGTCGTCTTTTAGATCACAAGCTTTGAACGTTAGCAAAATTATG10.2717391304347826No Hit
AATAAATTAAGAAGAGATCAAGAACTAATCGCAAATCTTAATTCAAACAA10.2717391304347826No Hit
AAAAAGAAGAAGACATACCTGAGCATGAAGGAGAAGAGAAGGAGATGAGG10.2717391304347826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)