FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_VvHY5_ampPN94-B_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_VvHY5_ampPN94-B_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41644556
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC4923741.1823250078593706TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT429790.10320436601605261TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)