FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_PtrbZIP53_ampPtr-B_w2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_PtrbZIP53_ampPtr-B_w2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21281993
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3336641.567823088749254TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC894790.42044464538636017TruSeq Adapter, Index 2 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCG520820.24472332079049178TruSeq Adapter, Index 2 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)