FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_PtrBSD_ampPtr-B_w1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_PtrBSD_ampPtr-B_w1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15020439
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC1950471.2985439373642806TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT258880.17235182007663025TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)