FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_LsWRKY_ampOkeechobee-B_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_LsWRKY_ampOkeechobee-B_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39275495
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC30528697.77296123193355TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1850360.4711232792864864TruSeq Adapter, Index 1 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCG850320.21650140883011149TruSeq Adapter, Index 1 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCGT483940.12321677931748537TruSeq Adapter, Index 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)