FastQCFastQC Report
Tue 26 Apr 2022
HJYV3BGXL_n01_LsLUX_ampOkeechobee-B_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYV3BGXL_n01_LsLUX_ampOkeechobee-B_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36103387
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC20473935.670916692663766TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1432250.39670793213944167TruSeq Adapter, Index 1 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCG496640.13756050090258845TruSeq Adapter, Index 1 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)