FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs9_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs9_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9333478
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT361030.386811861559003TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57950.037.5438411
ACACGTC59950.036.18124813
CACACGT61050.035.52933512
ACGTCTG60600.035.50237715
CGTCTGA60700.035.29892316
CACGTCT61150.035.2551914
GATCGGA59100.034.711861
AGCACAC63900.034.3578110
GAACTCC64100.033.01511821
TCCAGTC63350.032.91982725
GAGCACA66700.032.849549
CAGTCAC64350.032.64755627
CCAGTCA63900.032.6020526
TCACATG64400.032.2121230
GTCACAT65000.031.98246429
AGAGCAC68900.031.8329148
TCGGAAG68350.031.7811743
CTCGTAT53900.031.67117144
AGTCACA66550.031.33673528
CACATGT66700.031.16748631
Produced by FastQC (version 0.11.2)