FastQCFastQC Report
Tue 25 Apr 2017
HJYMHAFXX_n01_vs8_170421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJYMHAFXX_n01_vs8_170421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8570321
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT780680.9109110382213221TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG107600.040.95126311
CACGTCT109950.039.9957314
ACGTCTG109950.039.97572315
CGTCTGA110000.039.81756216
ACACGTC110950.039.7542113
CACACGT110900.039.69278712
AGCACAC113350.038.97093210
AGTTCCG108700.038.77591334
GAGCACA114800.038.6703349
CCGTATC100700.038.4921838
CAGTCAC113150.038.41744627
TATCTCG102500.037.94499241
GTTCCGT109750.037.94391635
TCCAGTC114100.037.94333625
GATCGGA113050.037.9074551
CCAGTCA114200.037.8908526
GTCACAG114100.037.86621529
TCCGTAT106700.037.8121237
CTCGTAT104750.037.7809844
GAACTCC116600.037.469421
Produced by FastQC (version 0.11.2)